Document Detail

Differential effects of methylphenidate and cocaine on GABA transmission in sensory thalamic nuclei.
MedLine Citation:
PMID:  23205768     Owner:  NLM     Status:  MEDLINE    
Methylphenidate (MPH) is widely used to treat children and adolescents diagnosed with attention deficit/hyperactivity disorder. Although MPH shares mechanistic similarities to cocaine, its effects on GABAergic transmission in sensory thalamic nuclei are unknown. Our objective was to compare cocaine and MPH effects on GABAergic projections between thalamic reticular and ventrobasal (VB) nuclei. Mice (P18-30) were subjected to binge-like cocaine and MPH acute and sub-chronic administrations. Cocaine and MPH enhanced hyperlocomotion, although sub-chronic cocaine-mediated effects were stronger than MPH effects. Cocaine and MPH sub-chronic administration altered paired-pulse and spontaneous GABAergic input differently. The effects of cocaine on evoked paired-pulse GABA-mediated currents changed from depression to facilitation with the duration of the protocols used, while MPH induced a constant increase throughout the administration protocols. Thalamic reticular nucleus GAD67 and VB Ca(V) 3.1 protein levels were measured using western blot to better understand their link to increased GABA release. Both proteins were increased by sub-chronic administration of cocaine. MPH showed effects on GABAergic transmission that seems less disruptive than cocaine. Unique effects of cocaine on postsynaptic VB calcium currents might explain deleterious cocaine effects on sensory thalamic nuclei. These results suggest that cocaine and MPH produced distinct presynaptic alterations on GABAergic transmission.
Belén Goitia; Mariana Raineri; Laura E González; José L Rozas; Edgar Garcia-Rill; Verónica Bisagno; Francisco J Urbano
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Publication Detail:
Type:  Journal Article; Research Support, N.I.H., Extramural; Research Support, Non-U.S. Gov't     Date:  2013-01-07
Journal Detail:
Title:  Journal of neurochemistry     Volume:  124     ISSN:  1471-4159     ISO Abbreviation:  J. Neurochem.     Publication Date:  2013 Mar 
Date Detail:
Created Date:  2013-02-18     Completed Date:  2013-04-19     Revised Date:  2014-03-03    
Medline Journal Info:
Nlm Unique ID:  2985190R     Medline TA:  J Neurochem     Country:  England    
Other Details:
Languages:  eng     Pagination:  602-12     Citation Subset:  IM    
Copyright Information:
© 2012 International Society for Neurochemistry.
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MeSH Terms
Blotting, Western
Central Nervous System Stimulants / toxicity*
Cocaine / toxicity*
Methylphenidate / toxicity*
Mice, Inbred C57BL
Patch-Clamp Techniques
Synaptic Transmission / drug effects*
Thalamic Nuclei / drug effects*,  metabolism
gamma-Aminobutyric Acid / metabolism*
Grant Support
P20 GM103425/GM/NIGMS NIH HHS; P20 GM103425-09/GM/NIGMS NIH HHS; R01 NS020246/NS/NINDS NIH HHS; R01 NS020246-25/NS/NINDS NIH HHS
Reg. No./Substance:
0/Central Nervous System Stimulants; 207ZZ9QZ49/Methylphenidate; 56-12-2/gamma-Aminobutyric Acid; I5Y540LHVR/Cocaine

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine

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