Document Detail

Differential effects of lysolipids on steroid synthesis in cells expressing endogenous LPA2 receptor.
MedLine Citation:
PMID:  15716590     Owner:  NLM     Status:  MEDLINE    
Incubation of ovarian luteal cells with the bioactive lipid mediator lysophosphatidic acid (LPA) for 180 min abolishes gonadotropin-induced steroid production with no attenuation of the cyclic AMP accumulation. Treatment with the lysolipid also diminishes [14C]steroid production in cells preloaded with either [14C]cholesterol or [14C]acetate. Neither the expression of steroidogenic acute regulatory (StAR) protein nor in vitro steroid synthesis is affected in isolated mitochondrial fractions. The LPA-induced attenuation of steroid production occurs only in the mid-cycle corpus luteum and is associated with a transient endogenous expression of mRNA for the lysophosphatidic acid A2 (LPA2) receptor (with no concomitant changes in the expression of LPA1 receptor). Expression of LPA2 is accompanied by LPA-induced sphingosine-1-phosphate (S1P) production. Because luteal cells, in the presence of the sphingosine kinase inhibitor dihydrosphingosine, can overcome the inhibitory effects of LPA on steroid synthesis, we suggest the possible requirement of intracellular S1P production. Interestingly, no LPA-induced inhibition of 8Br-cAMP-stimulated progesterone synthesis can be detected in Leydig tumor cell line MA10 cells expressing only LPA2 receptor. Surprisingly, however, exogenous S1P inhibits agonist-stimulated progesterone in both cell types by inhibiting cyclic AMP accumulation, suggesting different mechanisms of action.
Lygia T Budnik; Bärbel Brunswig-Spickenheier
Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't     Date:  2005-02-16
Journal Detail:
Title:  Journal of lipid research     Volume:  46     ISSN:  0022-2275     ISO Abbreviation:  J. Lipid Res.     Publication Date:  2005 May 
Date Detail:
Created Date:  2005-04-18     Completed Date:  2005-11-01     Revised Date:  2006-11-15    
Medline Journal Info:
Nlm Unique ID:  0376606     Medline TA:  J Lipid Res     Country:  United States    
Other Details:
Languages:  eng     Pagination:  930-41     Citation Subset:  IM    
Institute for Hormone and Fertility Research, Anatomy I, University Hospital Eppendorf, Martinistrasse 52, D-20246, Hamburg, Germany.
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MeSH Terms
Base Sequence
Cells, Cultured
Cholesterol / metabolism
Corpus Luteum / metabolism
DNA Primers
Lysophospholipids / metabolism,  pharmacology*
Progesterone / antagonists & inhibitors*
Receptors, Lysophosphatidic Acid / agonists,  metabolism*
Sphingosine / analogs & derivatives,  metabolism
Reg. No./Substance:
0/DNA Primers; 0/Lysophospholipids; 0/Receptors, Lysophosphatidic Acid; 123-78-4/Sphingosine; 22002-87-5/lysophosphatidic acid; 26993-30-6/sphingosine 1-phosphate; 57-83-0/Progesterone; 57-88-5/Cholesterol

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine

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