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Differential effects of intranasal vaccination with recombinant NcPDI in different mouse models of Neospora caninum Infection.
MedLine Citation:
PMID:  23046358     Owner:  NLM     Status:  Publisher    
Abstract/OtherAbstract:
In this study, mice were vaccinated intranasally with recombinant N. caninum protein disulfide isomerase (NcPDI) emulsified in cholera toxin (CT) or cholera toxin subunit B (CTB) from Vibrio cholerae. The effects of vaccination were assessed in the murine non-pregnant model and the fetal infection model, respectively. In the non-pregnant mice, previous results were confirmed, in that intranasal vaccination with recNcPDI in CT was highly protective, and low cerebral parasite loads were noted upon real time PCR analysis. Protection was accompanied by an IgG1-biased anti-NcPDI response upon IFNection and significantly increased expression of Th2 (IL-4 / IL-10) and IL-17 transcripts in spleen compared to corresponding values in mice treated with CT only. However, vaccination with recNcPDI in CT did not induce significant protection in dams and their offspring. In the dams, increased splenic Th1 (IFN-γ / IL12) and Th17 mRNA expression was detected. No protection was noted in the groups vaccinated with recNcPDI emulsified in CTB. Thus, vaccination with recNcPDI in CT in non-pregnant mice followed by challenge Infection induced a protective Th2-biased immune response, while in the pregnant mouse model the same vaccine formulation resulted in a Th1-biased Inflammatory response and failed to protect dams and their progeny. © 2012 Blackwell Publishing Ltd.
Authors:
Karim Debache; Andrew Hemphill
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Publication Detail:
Type:  JOURNAL ARTICLE     Date:  2012-10-10
Journal Detail:
Title:  Parasite immunology     Volume:  -     ISSN:  1365-3024     ISO Abbreviation:  Parasite Immunol.     Publication Date:  2012 Oct 
Date Detail:
Created Date:  2012-10-10     Completed Date:  -     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  7910948     Medline TA:  Parasite Immunol     Country:  -    
Other Details:
Languages:  ENG     Pagination:  -     Citation Subset:  -    
Copyright Information:
© 2012 Blackwell Publishing Ltd.
Affiliation:
Institute of Parasitology, Vetsuisse Faculty, University of Berne, Länggass-Strasse 122, CH-3012, Berne, Switzerland.
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