Document Detail

Differential effects of insulin-like growth factor-I and gonadotropins on the proliferative activity of two subgroups of granulosa cells: cumulus oophorus and mural granulosa cells.
MedLine Citation:
PMID:  11334915     Owner:  NLM     Status:  MEDLINE    
OBJECTIVE: To evaluate the physiological role of insulin-like growth factor-I (IGFI) and its interaction with gonadotropins in cell replication of two types of granulosa cells (cumulus oophorus, CC) and mural granulosa cells (MGC). DESIGN: Controlled randomized study of the action and interaction of gonadotropins and IGFI on granulosa cell replication in the rat. SETTING: A university reproductive biology laboratory. INTERVENTION(S): The study examined the in vivo treatment with gonadotropins or an analogue of IGFI, long Arg3-insulin-like growth factor (LR3-IGFI), which does not bind to IGFI-binding proteins. MAIN OUTCOME MEASURE(S): Granulosa cell replication was evaluated by the use of 3H-thymidine incorporation. RESULT(S): It was noted that the CC replicate much faster than the MGC. These two types of granulosa cells have very different dose response curves to IGFI. Differential responses were seen in animal cells exposed to long Arg3-insulin-like growth factor in vivo and then exposed to FSH and LH in vitro. CONCLUSION(S): Although murine granulosa cells show proliferative activity when they are exposed to IGFI, the two types, CC and MGC, respond differently. IGFI is not the sole mediator of the action of FSH, and these two chemicals may act independently or in concert.
F Khamsi; S Roberge
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Publication Detail:
Type:  Clinical Trial; Journal Article; Randomized Controlled Trial    
Journal Detail:
Title:  Fertility and sterility     Volume:  75     ISSN:  0015-0282     ISO Abbreviation:  Fertil. Steril.     Publication Date:  2001 May 
Date Detail:
Created Date:  2001-05-03     Completed Date:  2001-06-07     Revised Date:  2003-11-14    
Medline Journal Info:
Nlm Unique ID:  0372772     Medline TA:  Fertil Steril     Country:  United States    
Other Details:
Languages:  eng     Pagination:  997-1003     Citation Subset:  IM    
Toronto Fertility Sterility Institute, Toronto General Hospital, University Health Network, Toronto, Ontario, Canada.
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MeSH Terms
Cell Division / drug effects,  physiology
Chorionic Gonadotropin / pharmacology*,  physiology
Diethylstilbestrol / administration & dosage,  pharmacology
Dose-Response Relationship, Drug
Follicle Stimulating Hormone / pharmacology
Granulosa Cells / cytology,  drug effects*,  physiology
Insulin-Like Growth Factor I / analogs & derivatives*,  pharmacology*,  physiology*
Luteinizing Hormone / pharmacology
Random Allocation
Rats, Long-Evans
Rats, Sprague-Dawley
Reg. No./Substance:
0/Chorionic Gonadotropin; 0/LR(3)IGF-I; 56-53-1/Diethylstilbestrol; 67763-96-6/Insulin-Like Growth Factor I; 9002-67-9/Luteinizing Hormone; 9002-68-0/Follicle Stimulating Hormone

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine

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