| Differential effects of hyperthermia on macrophage interleukin-6 and tumor necrosis factor-alpha expression. | |
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MedLine Citation:
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PMID: 8178969 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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The pyrogenic cytokines tumor necrosis factor-alpha (TNF-alpha) and interleukin-6 (IL-6) appear in the circulation during infections and injuries, but TNF-alpha and IL-6 are regulated differently in macrophages. We compared the effects of elevated temperatures within the usual febrile range on the expression of TNF-alpha and IL-6 in vitro in lipopolysaccharide (LPS)-stimulated human macrophages derived from peripheral blood monocytes (HuMoM phi). During an 18-h incubation at 37 degrees C with 5 ng/ml LPS, these cells released 5,030 +/- 1,460 pg TNF-alpha/10(6) cells (means +/- SE) and 1,380 +/- 280 pg IL-6/10(6) cells. In LPS-stimulated HuMoM phi incubated at 40 degrees C, TNF-alpha release was almost completely inhibited (76 +/- 76 pg TNF-alpha/10(6) cells; P < 0.01 compared with LPS-stimulated HuMoM phi at 37 degrees C), but release of IL-6 was preserved (1,600 +/- 780 pg IL-6/10(6) cells). Western and Northern analyses showed that levels of TNF-alpha mRNA and cell-associated and secreted TNF-alpha protein were decreased, but IL-6 expression was unchanged at 40 degrees C in LPS-stimulated macrophages. Incubating HuMoM phi at 40 degrees did not alter their viability after 18 h but induced a 75-fold increase in levels of the inducible heat-shock protein 72 (HSP-72) mRNA in the face of a 56% inhibition in total protein synthesis. Our results show that IL-6 expression persisted at incubation temperatures in the upper end of the physiological range that induced heat shock and attenuated the expression of functionally active TNF-alpha in LPS-stimulated HuMoM phi. |
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Authors:
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J E Ensor; S M Wiener; K A McCrea; R M Viscardi; E K Crawford; J D Hasday |
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Publication Detail:
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Type: Journal Article; Research Support, Non-U.S. Gov't; Research Support, U.S. Gov't, Non-P.H.S.; Research Support, U.S. Gov't, P.H.S. |
Journal Detail:
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Title: The American journal of physiology Volume: 266 ISSN: 0002-9513 ISO Abbreviation: Am. J. Physiol. Publication Date: 1994 Apr |
Date Detail:
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Created Date: 1994-06-09 Completed Date: 1994-06-09 Revised Date: 2007-11-14 |
Medline Journal Info:
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Nlm Unique ID: 0370511 Medline TA: Am J Physiol Country: UNITED STATES |
Other Details:
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Languages: eng Pagination: C967-74 Citation Subset: IM |
Affiliation:
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Department of Medicine, University of Maryland School of Medicine, Baltimore. |
Export Citation:
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APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
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Adolescent Adult Fever / metabolism*, pathology Heat-Shock Proteins / metabolism Humans Interleukin-6 / metabolism* Lipopolysaccharides / pharmacology Macrophages / metabolism* Temperature Tumor Necrosis Factor-alpha / metabolism* |
| Grant Support | |
ID/Acronym/Agency:
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R29-CA52741/CA/NCI NIH HHS; R29-HL44853/HL/NHLBI NIH HHS |
| Chemical | |
Reg. No./Substance:
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0/Heat-Shock Proteins; 0/Interleukin-6; 0/Lipopolysaccharides; 0/Tumor Necrosis Factor-alpha |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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