Document Detail

Differential effects of heat shock on translation of normal mRNAs in primary spermatocytes, elongated spermatids, and Sertoli cells in seminiferous tubule culture.
MedLine Citation:
PMID:  9056428     Owner:  NLM     Status:  MEDLINE    
Male germ cells in mice develop normally at 32 degrees C and spermatogenesis is severely inhibited by higher temperatures, including abdominal temperature, 37 degrees C. To examine the effects of heat stress on protein synthesis in various testicular cell types, seminiferous tubules were cultured at 32 degrees or 37 degrees C for 70 min or 42.5 degrees or 44 degrees C for 10 min followed by incubation for 60 min at 32 degrees C. Cultures were labeled with [35S]methionine, and the proteins that are soluble in 4% trichloroacetic acid were analyzed by acid-urea polyacrylamide gel electrophoresis. This culture system preserves the cytoarchitecture of the seminiferous epithelium and avoids breaking late haploid cells (elongated spermatids) during tissue dissociation. Incorporation of [35S]methionine into histone H1t, the testis-specific subtype of histone H1, in pachytene primary spermatocytes (meiotic cells) was reduced by about 33-50% following incubation at 37 degrees and 42.5 degrees C and by >/=90% after incubation at 44 degrees C. In contrast, exposure to 37 degrees, 42.5 degrees, and 44 degrees C had minimal effects on incorporation into transition proteins 1 and 2 in elongated spermatids. To determine whether heat stress inhibits translational initiation, the distribution of several mRNAs in cytoplasmic extracts of cultured tubules was analyzed by sucrose gradients and Northern blots. Exposure to 37 degrees and 44 degrees C produces incremental reductions in the size of polysomes translating H1t mRNA in pachytene spermatocytes and the sulfated glycoprotein 2 mRNA in Sertoli cells, the somatic cell type in the germinal epithelium. Neither 37 degrees nor 44 degrees C reduces the size or proportion of polysomal protamine 2 mRNA in elongated spermatids. These results demonstrate that the initiation of translation in pachytene spermatocytes and Sertoli cells is inhibited by exposure to abdominal temperature and that elongated spermatids are much more resistant to thermal stress.
L Cataldo; M A Mastrangelo; K C Kleene
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Publication Detail:
Type:  Journal Article; Research Support, U.S. Gov't, Non-P.H.S.    
Journal Detail:
Title:  Experimental cell research     Volume:  231     ISSN:  0014-4827     ISO Abbreviation:  Exp. Cell Res.     Publication Date:  1997 Feb 
Date Detail:
Created Date:  1997-03-20     Completed Date:  1997-03-20     Revised Date:  2006-11-15    
Medline Journal Info:
Nlm Unique ID:  0373226     Medline TA:  Exp Cell Res     Country:  UNITED STATES    
Other Details:
Languages:  eng     Pagination:  206-13     Citation Subset:  IM    
Department of Biology, University of Massachusetts, Boston, 100 Morrissey Boulevard, Boston, Massachusetts, 02125-3393, USA.
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MeSH Terms
Centrifugation, Density Gradient
Chromosomal Proteins, Non-Histone / biosynthesis
Culture Techniques
Glycoproteins / genetics
Heat-Shock Response*
Histones / biosynthesis,  genetics
Methionine / metabolism
Molecular Chaperones*
Protamines / genetics
Protein Biosynthesis*
RNA, Messenger / analysis,  genetics
Seminiferous Tubules
Sertoli Cells / metabolism*
Spermatids / metabolism*
Spermatocytes / metabolism*
Reg. No./Substance:
0/Chromosomal Proteins, Non-Histone; 0/Clu protein, mouse; 0/Clusterin; 0/Glycoproteins; 0/Histones; 0/Molecular Chaperones; 0/Protamines; 0/RNA, Messenger; 0/protamine 2; 0/spermatid transition proteins; 63-68-3/Methionine

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