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Differential effects of formaldehyde exposure on the cell influx and vascular permeability in a rat model of allergic lung inflammation.
MedLine Citation:
PMID:  20685386     Owner:  NLM     Status:  Publisher    
Exposure to air pollutants such as formaldehyde (FA) leads to inflammation, oxidative stress and immune-modulation in the airways and is associated with airway inflammatory disorders such as asthma. The purpose of our study was to investigate the effects of exposure to FA on the allergic lung inflammation. The hypothesized link between reactive oxygen species and the effects of FA was also studied. To do so, male Wistar rats were exposed to FA inhalation (1%, 90min daily) for 3 days, and subsequently sensitized with ovalbumin (OVA)-alum by subcutaneous route. One week later the rats received another OVA-alum injection by the same route (booster). Two weeks later the rats were challenged with aerosolized OVA. The OVA challenge of rats upon FA exposure induced an elevated release of LTB(4), TXB(2), IL-1beta, IL-6 and VEGF in lung cells, increased phagocytosis and lung vascular permeability, whereas the cell recruitment into lung was reduced. FA inhalation induced the oxidative burst and the nitration of proteins in the lung. Vitamins C, E and apocynin reduced the levels of LTB(4) in BAL-cultured cells of the FA and FA/OVA groups, but increased the cell influx into the lung of the FA/OVA rats. In OVA-challenged rats, the exposure to FA was associated to a reduced lung endothelial cells expression of ICAM-1 (intercellular cell adhesion molecule 1). In conclusion, our findings suggest that FA down regulate the cellular migration into the lungs after an allergic challenge and increase the ability of resident lung cells likely macrophages to generate inflammatory mediators, explaining the increased lung vascular permeability. Our data are indicative that the actions of FA involve mechanisms related to endothelium-leukocyte interactions and oxidative stress, as far as the deleterious effects of this air pollutant on airways are concerned.
Adriana Lino-Dos-Santos-Franco; Helori Vanni Domingos; Ana Paula Ligeiro de Oliveira; Ana Cristina Breithaupt-Faloppa; Jean Pierre Schatzmann Peron; Simone Bolonheis; Marcelo Nicolas Muscará; Ricardo Martins Oliveira-Filho; B Boris Vargaftig; Wothan Tavares-de-Lima
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Publication Detail:
Type:  JOURNAL ARTICLE     Date:  2010-5-31
Journal Detail:
Title:  Toxicology letters     Volume:  -     ISSN:  1879-3169     ISO Abbreviation:  -     Publication Date:  2010 May 
Date Detail:
Created Date:  2010-8-5     Completed Date:  -     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  7709027     Medline TA:  Toxicol Lett     Country:  -    
Other Details:
Languages:  ENG     Pagination:  -     Citation Subset:  -    
Copyright Information:
Copyright © 2010. Published by Elsevier Ireland Ltd.
Departament of Pharmacology, Institute of Biomedical Sciences, University of São Paulo, São Paulo, Brazil.
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