Document Detail


Differential effects of ethanol on gamma-aminobutyric acid-A receptor-mediated synaptic currents in congenic strains of inbred long and short-sleep mice.
MedLine Citation:
PMID:  15365296     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
BACKGROUND: Ethanol enhances gamma-aminobutyric acid (GABA)A receptor-mediated responses in the brain, and this enhancement is greater in a mouse line behaviorally more sensitive to ethanol (long sleep) than in a line (short sleep) behaviorally less ethanol sensitive (assayed by loss of righting; sleep time). Quantitative trait locus (QTL) analysis of inbred long sleep (ILS) and inbred short sleep (ISS) phenotypes revealed four chromosomal regions (Lore1, Lore2, Lore4, and Lore5) that together account for approximately 50% of ethanol-induced sleep-time variance. Congenic strains were generated, each of which is homozygous for one of four ISS Lore QTLs on the ILS background. These congenic mouse strains are ideally suited for asking which QTL regions might correlate with other phenotypes that differ between ILS and ISS mice. Here we used the congenics to investigate altered GABAA responses to ethanol. METHODS: Evoked GABAA receptor-mediated inhibitory postsynaptic currents (IPSCs) were measured by whole-cell voltage-clamp recording procedures in CA1 pyramidal neurons in hippocampal brain slices. RESULTS: GABAA IPSC responses in hippocampal brain slices from ILS mice were significantly enhanced by 80 mM ethanol, whereas those from ISS mice were not affected. ILS.Lore2 and ILS.Lore5 congenic strains were significantly enhanced by 80 mM ethanol, similar to the background (control) ILS mice. However, ethanol had no significant effect on GABAA responses in ILS.Lore1 and ILS.Lore4 congenic mice, similar to the ISS mice, thus reflecting the influence of ISS alleles on the ILS phenotype. CONCLUSIONS: Our results suggest that alleles located in the Lore1 and Lore4 QTL regions confer ethanol sensitivity of GABAA receptor-mediated IPSCs. Thus, for these QTLs, GABAA IPSCs may represent an endophenotype of sedative/hypnotic sensitivity to ethanol. Although the Lore2 and Lore5 QTL regions have a significant effect on sleep time, they do not play a significant role in the differential ethanol enhancement of GABAA IPSCs between ILS and ISS mice.
Authors:
William R Proctor; Peter H Wu; Beth Bennett; Thomas E Johnson
Related Documents :
8984016 - Scintigraphic measurement of gastric emptying and ultrasonographic assessment of antral...
22366906 - Incidence of retinal breaks in eyes undergoing 23-gauge or 20-gauge vitrectomy with ind...
24637426 - A neuron-glia interaction involving gaba transaminase contributes to sleep loss in slee...
21872586 - Dopamine antagonists and brief vision distinguish lens-induced- and form-deprivation-in...
20075676 - An exploratory study of factors influencing glaucoma treatment adherence.
23853566 - Heart rate variability in sleep-related migraine without aura.
Publication Detail:
Type:  Comparative Study; Journal Article; Research Support, U.S. Gov't, Non-P.H.S.; Research Support, U.S. Gov't, P.H.S.    
Journal Detail:
Title:  Alcoholism, clinical and experimental research     Volume:  28     ISSN:  0145-6008     ISO Abbreviation:  Alcohol. Clin. Exp. Res.     Publication Date:  2004 Sep 
Date Detail:
Created Date:  2004-09-14     Completed Date:  2004-12-16     Revised Date:  2007-11-14    
Medline Journal Info:
Nlm Unique ID:  7707242     Medline TA:  Alcohol Clin Exp Res     Country:  United States    
Other Details:
Languages:  eng     Pagination:  1277-83     Citation Subset:  IM    
Affiliation:
Veterans Affairs Eastern Colorado Health Care System, Denver, Colorado, USA. bill.proctor@uchsc.edu
Export Citation:
APA/MLA Format     Download EndNote     Download BibTex
MeSH Terms
Descriptor/Qualifier:
Animals
Ethanol / pharmacology*
Male
Mice
Mice, Congenic
Receptors, GABA-A / genetics,  physiology*
Synapses / drug effects*,  genetics,  physiology
Synaptic Transmission / drug effects*,  genetics,  physiology
Grant Support
ID/Acronym/Agency:
KO2-AA00195/AA/NIAAA NIH HHS; P50-AA 03527/AA/NIAAA NIH HHS; R01-AA08940/AA/NIAAA NIH HHS
Chemical
Reg. No./Substance:
0/Receptors, GABA-A; 64-17-5/Ethanol

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


Previous Document:  Images in geriatric cardiology. Usefulness of live three-dimensional transthoracic echocardiography ...
Next Document:  Ethanol inhibits gap-junctional coupling between P19 cells.