Document Detail


Differential effects of electrolytic and chemical hypothalamic lesions on LH pulses in rats.
MedLine Citation:
PMID:  3056029     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
Electrolytic lesions of the arcuate nucleus were made in anesthetized adult castrated male rats. Luteinizing hormone (LH) pulse frequency averaged 2.4 pulses/h in controls but declined to a mean of 0.5 pulses/h in rats with bilateral damage to the arcuate nucleus. Because these lesions also damaged the median eminence, we tested the possibility that this disruption of LH secretion was due to coincidental damage to fibers of passage projecting to median eminence. Axon-sparing chemical lesions of the arcuate nucleus were made by intracranial injections of N-methyl-DL-aspartate (NMA) in anesthetized adult castrated rats. Mean LH pulse frequency was 2.3 and 2.5 pulses/h in control and NMA-injected rats, respectively. NMA injections destroyed arcuate neuronal cell bodies and produced a proliferation of glial cells within the nucleus. There was no apparent difference in the immunocytochemical staining intensity and distribution of luteinizing hormone-releasing hormone (LHRH) fibers in median eminence in rats receiving NMA or sham injections. These results suggest that the disruptive effects of electrolytic lesions of the arcuate nucleus on pulsatile LH secretion are a result of coincidental damage to LHRH neuronal projections to the median eminence and that neuronal cell bodies within the arcuate nucleus are not necessary for normal pulsatile LH secretion in male rats.
Authors:
C L Sisk; A A Nunez; M M Thebert
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Publication Detail:
Type:  Comparative Study; Journal Article; Research Support, U.S. Gov't, P.H.S.    
Journal Detail:
Title:  The American journal of physiology     Volume:  255     ISSN:  0002-9513     ISO Abbreviation:  Am. J. Physiol.     Publication Date:  1988 Nov 
Date Detail:
Created Date:  1988-12-09     Completed Date:  1988-12-09     Revised Date:  2007-11-14    
Medline Journal Info:
Nlm Unique ID:  0370511     Medline TA:  Am J Physiol     Country:  UNITED STATES    
Other Details:
Languages:  eng     Pagination:  E583-90     Citation Subset:  IM    
Affiliation:
Neuroscience Program, Michigan State University, East Lansing 48824.
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MeSH Terms
Descriptor/Qualifier:
Animals
Arcuate Nucleus / drug effects,  injuries,  physiology*
Aspartic Acid / analogs & derivatives
Electrolysis
Luteinizing Hormone / secretion*
Male
N-Methylaspartate
Periodicity
Rats
Rats, Inbred Strains
Grant Support
ID/Acronym/Agency:
HD-21588/HD/NICHD NIH HHS; MH-37877/MH/NIMH NIH HHS
Chemical
Reg. No./Substance:
56-84-8/Aspartic Acid; 6384-92-5/N-Methylaspartate; 9002-67-9/Luteinizing Hormone

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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