| Differential effects of Heparitinase I and Heparitinase III on endothelial tube formation in vitro. | |
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MedLine Citation:
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PMID: 20599743 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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Heparan sulfate proteoglycans (HSPGs) play vital roles in many steps of angiogenesis under physiological and pathological conditions. HSPGs on endothelial cell surfaces act as co-receptors for a variety of pro-angiogenic growth factors such as FGF and VEGF and anti-angiogenic factors such as endostatin. However, the fine structural requirements of these binding interactions are dependent on the sulfation patterns of HSPGs. Previous studies have shown that Heparitinases, heparin lyases isolated from Flavobacterium heparinum, can cleave heparan sulfate chains. These enzymes have been shown to reduce tumor-derived neovascularization in vivo in mice. However, the results from these experiments could not conclusively pinpoint the origin of the HS fragments. Thus, in this study we utilized an in vitro assay to assess the differential effects of Heparitinase I (Hep I) and Heparitinase III (Hep III) on endothelial tube formation. Hep III was found to be a more potent inhibitor of tube formation than Hep I. In conclusion, differential cleavage of endothelial cell surface bound HS can affect the extent of inhibition of tube formation. |
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Authors:
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Karthik Raman; Balagurunathan Kuberan |
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Publication Detail:
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Type: Journal Article; Research Support, N.I.H., Extramural; Research Support, Non-U.S. Gov't Date: 2010-06-17 |
Journal Detail:
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Title: Biochemical and biophysical research communications Volume: 398 ISSN: 1090-2104 ISO Abbreviation: Biochem. Biophys. Res. Commun. Publication Date: 2010 Jul |
Date Detail:
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Created Date: 2010-07-26 Completed Date: 2010-08-09 Revised Date: 2011-08-01 |
Medline Journal Info:
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Nlm Unique ID: 0372516 Medline TA: Biochem Biophys Res Commun Country: United States |
Other Details:
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Languages: eng Pagination: 191-3 Citation Subset: IM |
Copyright Information:
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Copyright (c) 2010 Elsevier Inc. All rights reserved. |
Affiliation:
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Department of Bioengineering, University of Utah, Salt Lake City, UT 84112, USA. |
Export Citation:
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| MeSH Terms | |
Descriptor/Qualifier:
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Angiogenesis Inhibitors
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chemistry,
isolation & purification,
pharmacology* Animals Cattle Cells, Cultured Endothelial Cells / drug effects* Flavobacterium / enzymology* Neovascularization, Physiologic / drug effects* Polysaccharide-Lyases / chemistry, isolation & purification, pharmacology* |
| Grant Support | |
ID/Acronym/Agency:
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GM075168/GM/NIGMS NIH HHS; R01 GM075168-04/GM/NIGMS NIH HHS; R01 GM075168-05/GM/NIGMS NIH HHS |
| Chemical | |
Reg. No./Substance:
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0/Angiogenesis Inhibitors; EC 4.2.2.-/Polysaccharide-Lyases; EC 4.2.2.8/heparitinsulfate lyase |
| Comments/Corrections | |
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