| Differential effects of ET-1, ET-2, and ET-3 on pancreatic microcirculation, tissue integrity, and inflammation. | |
| | |
MedLine Citation:
|
PMID: 11414314 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
|
The differential effects of endothelin-1, -2, and -3 (ET-1, ET-2, and ET-3) on pancreatic microcirculation, pancreatic tissue integrity, and an initial inflammatory response, which are three distinct characteristics of acute necrotizing pancreatitis, were investigated in a dose-dependent manner in rats using in vivo microscopy. Red blood cell (RBC) velocity and functional capillary density (FCD) were estimated after topical superfusion of the pancreas with ET-1, ET-2, and ET-3 (100, 10, 1 pmol), revealing that ET-1 (100, 10, 1 pmol) or high ET-2 (100 pmol) and ET-3 (100 pmol) cause a dose-related deterioration of exocrine nutritive pancreatic blood flow. Analysis of pancreatic exocrine tissue damage employing the Spormann score displayed that the ET-mediated microcirculatory impairment was paralleled by dose-dependent tissue damage, which was significant compared to the control group (topical superfusion with 1 ml, saline solution 0.9%). Estimation of pancreatic postcapillary leukocyte accumulation by histomorphologically counting choracetate esterase (CAE) stained leukocytes in 50 high-power fields per animal demonstrated a significant increase in postcapillary accumulation of white blood cells, after topical administration of ET-1, ET-2, and ET-3 compared to controls. In contrast to ET-caused effects on microcirculation and tissue impairment, quantitative analysis of postcapillary leukocyte accumulation revealed the most pronounced effect after ET-2 administration but not after ET-1 administration. This demonstrates that ET-1, ET-2, and ET-3 are all able to mediate microcirculatory impairment, tissue damage, and inflammation. However, ET-3-induced damaging effects are less pronounced, while ET-1 most severely alters microcirculation and ET-2 preferentially induces leukocyte-dependent inflammation. |
| | |
Authors:
|
T Plusczyk; B Bersal; M D Menger; G Feifel |
Related Documents
:
|
15914284 - Redox status of dogs with non-hodgkin lymphomas. an esr study. 10795554 - Frequency of pancreatitis after endoscopic retrograde cholangiopancreatography with iop... 22533724 - Kallikrein gene transfer induces angiogenesis and further improves regional cerebral bl... 2655714 - Quantitation and immunohistochemical localization of cathepsins e and d in rat tissues ... 17354784 - Particle filtering for nonlinear bold signal analysis. 7381674 - Tubular duplication with autonomous blood supply: resection with preservation of adjace... |
Publication Detail:
|
Type: Comparative Study; Journal Article |
Journal Detail:
|
Title: Digestive diseases and sciences Volume: 46 ISSN: 0163-2116 ISO Abbreviation: Dig. Dis. Sci. Publication Date: 2001 Jun |
Date Detail:
|
Created Date: 2001-06-20 Completed Date: 2001-07-19 Revised Date: 2006-11-15 |
Medline Journal Info:
|
Nlm Unique ID: 7902782 Medline TA: Dig Dis Sci Country: United States |
Other Details:
|
Languages: eng Pagination: 1343-51 Citation Subset: AIM; IM |
Affiliation:
|
Department of General Surgery, University of Saarland, Homburg/Saar, Germany. |
Export Citation:
|
APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
|
Animals Dose-Response Relationship, Drug Endothelin-1 / pharmacology, physiology* Endothelin-2 / pharmacology, physiology* Endothelin-3 / pharmacology, physiology* Male Microcirculation / drug effects Pancreas / blood supply*, drug effects, immunology, pathology Pancreatitis, Acute Necrotizing / etiology, immunology Rats Rats, Inbred Lew |
| Chemical | |
Reg. No./Substance:
|
0/Endothelin-1; 0/Endothelin-2; 0/Endothelin-3 |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
Previous Document: Diagnostic accuracy of fecal elastase 1 assay in patients with pancreatic maldigestion or intestinal...
Next Document: Directed evolution and the creation of enantioselective biocatalysts.