Document Detail

Differential effect of the shape of calcium alginate matrices on the physiology of immobilized neuroblastoma N2a and Vero cells: a comparative study.
MedLine Citation:
PMID:  17719216     Owner:  NLM     Status:  MEDLINE    
In order to investigate the effect of cell immobilization in calcium alginate gels on cell physiology, we immobilized Vero or N2a neuroblastoma cells in gels shaped either as spherical beads or as thin membrane layers. Throughout a culture period of 4 weeks cell viability, RNA and cytoplasmic calcium concentration and glutathione accumulation were assayed by fluorescence microscopy after provision of an appropriate dye. Non-elaborate culture conditions were applied throughout the experimental period in order to evaluate cell viability under less than optimal storage conditions. Vero cell proliferation was observed only in spherical beads, while N2a cell proliferation was observed in both configurations until the third week of culture. Increased [Ca2+]cyt could be associated with cell proliferation only when cells were immobilized in spherical beads, while a considerable decrease in the biosynthesis of reduced glutathione and RNA was observed in cells immobilized in thin membrane layers. The observed effects of the shape of the immobilization matrix may be due to differences in external mass transfer resistance. Therefore, depending on cell type, cell proliferation could have been promoted by either increased (Vero) or decreased (N2a) nutrient and oxygen flow to immobilized cells. The results of the present study could contribute to an improvement of immobilized cell sensor storability.
S Kintzios; I Yiakoumetis; G Moschopoulou; O Mangana; K Nomikou; A Simonian
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Publication Detail:
Type:  Comparative Study; Journal Article     Date:  2007-07-20
Journal Detail:
Title:  Biosensors & bioelectronics     Volume:  23     ISSN:  0956-5663     ISO Abbreviation:  Biosens Bioelectron     Publication Date:  2007 Nov 
Date Detail:
Created Date:  2007-10-22     Completed Date:  2008-02-26     Revised Date:  2009-07-14    
Medline Journal Info:
Nlm Unique ID:  9001289     Medline TA:  Biosens Bioelectron     Country:  England    
Other Details:
Languages:  eng     Pagination:  543-8     Citation Subset:  IM    
Laboratory of Plant Physiology, Faculty of Agricultural Biotechnology, Agricultural University of Athens, Greece. <>
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MeSH Terms
Alginates / chemistry,  pharmacology*
Cell Death / drug effects
Cell Line, Tumor
Cell Proliferation / drug effects
Cell Survival / drug effects
Cells, Immobilized / drug effects*
Cercopithecus aethiops
Glucuronic Acid / chemistry,  pharmacology
Hexuronic Acids / chemistry,  pharmacology
Microscopy, Fluorescence
Time Factors
Vero Cells
Reg. No./Substance:
0/Alginates; 0/Hexuronic Acids; 576-37-4/Glucuronic Acid; 9005-32-7/alginic acid

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine

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