Document Detail


Differential effect of ischemic and pharmacological preconditioning on PKC isoform translocation in adult rat cardiac myocytes.
MedLine Citation:
PMID:  12438767     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
The role of PKC isoforms in the protection of ischemic preconditioning remains controversial. The aim of the present study was to compare PKC translocation in ischemic and pharmacological preconditioning and to test the hypothesis that induction of the preconditioned state results in a sustained translocation of PKC during the following ischemic period. Isolated rat cardiac myocytes were subjected to established pre-conditioning protocols using either transient ischemia or alpha(1)-adrenergic stimulation. Translocation of PKC isoforms, -alpha, -delta and -epsilon to the particulate fraction during induction of preconditioning, post incubation or final sustained ischemia was assessed by immunoblotting. Ischemia alone caused the translocation of PKC-alpha and -epsilon from the soluble to the particulate fraction. All three PKC isoforms examined were translocated to the particulate fraction in response to stimulation with alpha(1)-adrenergic agonists or phorbol esters. Ischemic preconditioning resulted in the translocation of only the PKC-epsilon isoform while pharmacological precondi-tion-ing did not affect any of the isoforms. During the following sustained ischemic period, increased percentage of PKC-alpha and -epsilon isoforms associated with the particulate fraction was observed only for the pharmacologically preconditioned cells. It is concluded that PKC translocation during preconditioning or the following ischemic period is not essential for the mediation of protection of rat cardiomyocytes in vitro.
Authors:
Vasiliki Tsouka; Thomais Markou; Antigone Lazou
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Publication Detail:
Type:  Comparative Study; Journal Article    
Journal Detail:
Title:  Cellular physiology and biochemistry : international journal of experimental cellular physiology, biochemistry, and pharmacology     Volume:  12     ISSN:  1015-8987     ISO Abbreviation:  Cell. Physiol. Biochem.     Publication Date:  2002  
Date Detail:
Created Date:  2002-11-19     Completed Date:  2003-06-10     Revised Date:  2007-11-15    
Medline Journal Info:
Nlm Unique ID:  9113221     Medline TA:  Cell Physiol Biochem     Country:  Switzerland    
Other Details:
Languages:  eng     Pagination:  315-24     Citation Subset:  IM    
Copyright Information:
Copyright 2002 S. Karger AG, Basel
Affiliation:
Laboratory of Animal Physiology, Dept. of Zoology, School of Biology, Aristotle University of Thessaloniki, Greece.
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MeSH Terms
Descriptor/Qualifier:
Adrenergic alpha-Agonists / pharmacology*
Animals
Biological Transport / drug effects
Cell Size
Immunoblotting
Ischemic Preconditioning, Myocardial*
Isoenzymes / metabolism
Myocytes, Cardiac / drug effects,  enzymology*
Phenylephrine / pharmacology*
Phorbol Esters / pharmacology
Protein Kinase C / metabolism*
Rats
Rats, Wistar
Subcellular Fractions / enzymology
Chemical
Reg. No./Substance:
0/Adrenergic alpha-Agonists; 0/Isoenzymes; 0/Phorbol Esters; 59-42-7/Phenylephrine; EC 2.7.11.13/Protein Kinase C

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