| Differential down-regulation of insulin-sensitive protein kinase-C isoforms by 12-O-tetradecanoylphorbol-13-acetate in rat adipocytes and BC3H-1 myocytes. | |
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MedLine Citation:
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PMID: 8425487 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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In rat adipocytes, chronic incubation with 12-O-tetradecanoylphorbol-13-acetate (TPA) reduced immunoreactive protein kinase-C (PKC) beta, gamma, delta, and zeta isoforms by 40-60% and PKC alpha by 75%, but had little effect on PKC epsilon levels. In BC3H-1 myocytes, chronic TPA treatment had no effect on PKC beta, increased PKC zeta, and depleted PKC alpha. Acute treatment with insulin induced the translocation of PKC beta in the myocytes both before and after chronic TPA treatment, but had no acute effect on the alpha or zeta isoforms. In contrast, acute TPA treatment in the myocytes had little effect on PKC beta, but induced the rapid translocation of alpha and zeta. The differential effects of chronic TPA treatment on the down-regulation of PKC beta may explain why insulin continues to activate biological processes in TPA-treated BC3H-1 myocytes, but not in adipocytes. |
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Authors:
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M L Standaert; D R Cooper; H Hernandez; T P Arnold; R V Farese |
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Publication Detail:
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Type: Journal Article; Research Support, U.S. Gov't, Non-P.H.S.; Research Support, U.S. Gov't, P.H.S. |
Journal Detail:
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Title: Endocrinology Volume: 132 ISSN: 0013-7227 ISO Abbreviation: Endocrinology Publication Date: 1993 Feb |
Date Detail:
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Created Date: 1993-03-02 Completed Date: 1993-03-02 Revised Date: 2007-11-15 |
Medline Journal Info:
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Nlm Unique ID: 0375040 Medline TA: Endocrinology Country: UNITED STATES |
Other Details:
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Languages: eng Pagination: 689-92 Citation Subset: AIM; IM |
Affiliation:
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Research Service, J. A. Haley V.A. Hospital, Tampa, Florida 33612. |
Export Citation:
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APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
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Adipose Tissue
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drug effects,
enzymology* Animals Cell Line Cell Membrane / enzymology Cells, Cultured Cytosol / enzymology Insulin / pharmacology* Isoenzymes / metabolism* Kinetics Male Muscles / drug effects, enzymology* Protein Kinase C / metabolism* Rats Tetradecanoylphorbol Acetate / pharmacology* |
| Grant Support | |
ID/Acronym/Agency:
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DK-38079/DK/NIDDK NIH HHS |
| Chemical | |
Reg. No./Substance:
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0/Isoenzymes; 11061-68-0/Insulin; 16561-29-8/Tetradecanoylphorbol Acetate; EC 2.7.11.13/Protein Kinase C |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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