| Differential dopamine-induced prolactin mRNA levels in various prolactin-secreting cell (sub)populations. | |
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MedLine Citation:
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PMID: 1564425 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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We have examined the effects of dopamine on prolactin gene expression using quantitative in-situ hybridization histochemistry in different pituitary cell (sub)populations separated according to their density on a discontinuous Percoll gradient. Administration of dopamine resulted in a drastic reduction in hybridization of 35S-labelled DNA probe complementary to prolactin mRNA in total pituitary cells and in lactotrophs with low density. In contrast, dopamine significantly stimulated mRNA accumulation in prolactin-secreting cells with high density compared with other cell layers. The combined use of Percoll gradient and quantitative in-situ hybridization is a valuable and sensitive method with which to examine prolactin-secreting cell response to a given stimulation. Prolactin-secreting cells with high and low density clearly show functional heterogeneity in their response to dopamine. |
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Authors:
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M Kazemzadeh; B Velkeniers; P Herregodts; R Collumbien; E Finné; M P Derde; L Vanhaelst; E L Hooghe-Peters |
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Publication Detail:
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Type: Journal Article; Research Support, Non-U.S. Gov't |
Journal Detail:
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Title: The Journal of endocrinology Volume: 132 ISSN: 0022-0795 ISO Abbreviation: J. Endocrinol. Publication Date: 1992 Mar |
Date Detail:
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Created Date: 1992-05-19 Completed Date: 1992-05-19 Revised Date: 2006-11-15 |
Medline Journal Info:
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Nlm Unique ID: 0375363 Medline TA: J Endocrinol Country: ENGLAND |
Other Details:
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Languages: eng Pagination: 401-9 Citation Subset: IM |
Affiliation:
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Department of Pharmacology, Medical School, Free University of Brussels, Belgium. |
Export Citation:
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APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
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Animals Cells, Cultured Dopamine / physiology* Female Gene Expression / physiology* Nucleic Acid Hybridization Pituitary Gland, Anterior / cytology, physiology* Prolactin / genetics*, metabolism RNA, Messenger / analysis* Rats Rats, Inbred Strains |
| Chemical | |
Reg. No./Substance:
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0/RNA, Messenger; 9002-62-4/Prolactin |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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