Document Detail

Differential dopamine-induced prolactin mRNA levels in various prolactin-secreting cell (sub)populations.
MedLine Citation:
PMID:  1564425     Owner:  NLM     Status:  MEDLINE    
We have examined the effects of dopamine on prolactin gene expression using quantitative in-situ hybridization histochemistry in different pituitary cell (sub)populations separated according to their density on a discontinuous Percoll gradient. Administration of dopamine resulted in a drastic reduction in hybridization of 35S-labelled DNA probe complementary to prolactin mRNA in total pituitary cells and in lactotrophs with low density. In contrast, dopamine significantly stimulated mRNA accumulation in prolactin-secreting cells with high density compared with other cell layers. The combined use of Percoll gradient and quantitative in-situ hybridization is a valuable and sensitive method with which to examine prolactin-secreting cell response to a given stimulation. Prolactin-secreting cells with high and low density clearly show functional heterogeneity in their response to dopamine.
M Kazemzadeh; B Velkeniers; P Herregodts; R Collumbien; E Finné; M P Derde; L Vanhaelst; E L Hooghe-Peters
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  The Journal of endocrinology     Volume:  132     ISSN:  0022-0795     ISO Abbreviation:  J. Endocrinol.     Publication Date:  1992 Mar 
Date Detail:
Created Date:  1992-05-19     Completed Date:  1992-05-19     Revised Date:  2006-11-15    
Medline Journal Info:
Nlm Unique ID:  0375363     Medline TA:  J Endocrinol     Country:  ENGLAND    
Other Details:
Languages:  eng     Pagination:  401-9     Citation Subset:  IM    
Department of Pharmacology, Medical School, Free University of Brussels, Belgium.
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MeSH Terms
Cells, Cultured
Dopamine / physiology*
Gene Expression / physiology*
Nucleic Acid Hybridization
Pituitary Gland, Anterior / cytology,  physiology*
Prolactin / genetics*,  metabolism
RNA, Messenger / analysis*
Rats, Inbred Strains
Reg. No./Substance:
0/RNA, Messenger; 9002-62-4/Prolactin

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine

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