Document Detail

Differential distribution of nitric oxide synthase isoforms in the rostral ventrolateral medulla of the rat.
MedLine Citation:
PMID:  12711855     Owner:  NLM     Status:  MEDLINE    
We evaluated the distribution of nitric oxide synthase (NOS) isoforms in the rostral ventrolateral medulla (RVLM), the medullary origin of sympathetic neurogenic vasomotor tone, and the contribution of NOS III to the cardiovascular actions of endogenous NO in the RVLM. Adult Sprague-Dawley rats were used. Reverse transcription-polymerase chain reaction or Western blot analysis revealed that NOS I, II or III was expressed in the ventrolateral medulla at the mRNA or protein level under basal conditions. However, laser scanning confocal microscopic analysis of double-immunofluorescence images showed that whereas NOS I or II immunoreactivity colocalized with cells within the confines of the RVLM that stained positively with the neuronal marker, NeuN, NOS III immunoreactivity was associated primarily with blood vessels. Furthermore, bilateral microinjection into the RVLM of the selective NOS III inhibitor, N(5)-(1-iminoethyl)-L-ornithine, elicited minimal alterations in baseline systemic arterial pressure, heart rate or sympathetic vasomotor outflow in rats anesthetized with propofol. We conclude that whereas NOS I and II are present in neurons within the confines of the RVLM, NOS III is associated primarily with blood vessels. Our results further indicate that NOS III does not appear to contribute to the maintenance of basal sympathetic vasomotor outflows and arterial pressure by the endogenous NO at the RVLM.
Alice Y W Chang; Julie Y H Chan; Samuel H H Chan
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Publication Detail:
Type:  Comparative Study; Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Journal of biomedical science     Volume:  10     ISSN:  1021-7770     ISO Abbreviation:  J. Biomed. Sci.     Publication Date:    2003 May-Jun
Date Detail:
Created Date:  2003-04-24     Completed Date:  2004-01-21     Revised Date:  2006-11-15    
Medline Journal Info:
Nlm Unique ID:  9421567     Medline TA:  J Biomed Sci     Country:  Switzerland    
Other Details:
Languages:  eng     Pagination:  285-91     Citation Subset:  IM    
Copyright Information:
Copyright 2003 National Science Council, ROC and S. Karger AG, Basel
Center for Neuroscience, National Sun Yat-sen University, Kaohsiung 80424, Taiwan, ROC.
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MeSH Terms
Blotting, Western
Isoenzymes / chemistry
Medulla Oblongata / chemistry*
Microscopy, Confocal
Nitric Oxide Synthase / antagonists & inhibitors,  chemistry*,  metabolism
Rats / metabolism*
Rats, Sprague-Dawley
Reverse Transcriptase Polymerase Chain Reaction
Time Factors
Vasomotor System / metabolism
Reg. No./Substance:
0/Isoenzymes; EC Oxide Synthase

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