| Differential distribution of individual subunits of strongly inwardly rectifying potassium channels (Kir2 family) in rat brain. | |
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MedLine Citation:
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PMID: 15936845 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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Inwardly rectifying potassium (Kir) channels modulate cellular excitability, membrane potential, and secretion of neurotransmitters and hormones. Kir channels with the strongest inward rectification belong to the Kir2 family. In this report, polyclonal monospecific affinity-purified antibodies against the less conserved carboxy-terminal sequences of Kir2.1, Kir2.2, Kir2.3, and Kir2.4 were used to analyze the detailed distribution of all members of the Kir2 family in the rat central nervous system. Kir2 channel expression is detected in neurons but not in glial cells. Kir2 protein distribution confirms the basic mRNA localization pattern given by in situ hybridization. Kir2.1 is detected throughout the whole brain but in particular subsets of neurons with highest expression in olfactory bulb and superior colliculus. Kir2.2 immunoreactivity is primarily displayed in several forebrain nuclei, hypothalamus, cerebellum, and spinal cord. The Kir2.3 subunit is predominantly localized in olfactory bulb, basal ganglia, cortex, and cerebellar Purkinje cells. In contrast, Kir2.4-positive staining is detected at significantly lower levels in most neurons throughout the rat brain with highest expression in brainstem motoneurons. Thus, our data show a more widespread distribution of Kir2.4 than previously determined. In summary, the widespread presence of all four Kir2 channel subunits in the rat brain provides further evidence for their important role in central signal processing and neural transmission. |
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Authors:
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Harald Prüss; Christian Derst; Reinhard Lommel; Rüdiger W Veh |
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Publication Detail:
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Type: Journal Article; Research Support, Non-U.S. Gov't |
Journal Detail:
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Title: Brain research. Molecular brain research Volume: 139 ISSN: 0169-328X ISO Abbreviation: Brain Res. Mol. Brain Res. Publication Date: 2005 Sep |
Date Detail:
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Created Date: 2005-09-05 Completed Date: 2005-12-21 Revised Date: 2006-11-15 |
Medline Journal Info:
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Nlm Unique ID: 8908640 Medline TA: Brain Res Mol Brain Res Country: Netherlands |
Other Details:
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Languages: eng Pagination: 63-79 Citation Subset: IM |
Affiliation:
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Centrum für Anatomie der Charité, Universitätsklinikum der Humboldt-Universität Berlin, Philippstr. 12,10115 Berlin, Germany. |
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| MeSH Terms | |
Descriptor/Qualifier:
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Animals Antibodies / metabolism Brain / anatomy & histology*, metabolism* Immunohistochemistry In Situ Hybridization Neuroglia / cytology, metabolism Neurons / cytology, metabolism Potassium Channels, Inwardly Rectifying / genetics, metabolism* Protein Subunits / genetics, metabolism* Rats Rats, Wistar Tissue Distribution |
| Chemical | |
Reg. No./Substance:
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0/Antibodies; 0/Potassium Channels, Inwardly Rectifying; 0/Protein Subunits |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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