Document Detail


Differential distribution of cAMP-dependent protein kinase isoforms in the mantle of the bivalve mollusc Mytilus galloprovincialis.
MedLine Citation:
PMID:  19908149     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
Two different isoforms of the regulatory (R) subunit of cAMP-dependent protein kinase (PKA), named R(myt1) and R(myt2), had previously been identified in the sea mussel Mytilus galloprovincialis. R(myt1) and R(myt2) were differentially distributed in the various cell types comprising the mussel mantle. R(myt1) was found the only isoform to be present in the auxiliary cells of female follicles and the cubic epithelium of the middle fold of mantle edge. In contrast, only the R(myt2) isoform was detected in the subepithelial connective tissue, the endothelium of haemolymph vessels, the adipogranular and vesicular cells of reserve connective tissue, and the spermatozoa. Finally, both R(myt1) and R(myt2) coexist in some cell types but they show a different cellular localization: R(myt1) was localized in the cytoplasm whereas R(myt2) was mainly detected in the cell apical edge, cell periphery, cilia and sperm flagella. Interestingly, both R(myt1) and R(myt2) were absent in oocytes within the female gonadal follicles but, in contrast, they were highly expressed in follicle-released oocytes collected after spawning. Taken together the results show that mussel PKA isoforms are differentially distributed in the mantle cell types, which suggests that they are involved in the regulation of distinct cellular functions. On the other hand, the expression of R(myt1) and R(myt2) proteins is associated with the meiosis resumption of oocytes at the prophase I stage, which occurs in parallel to spawning.
Authors:
Jos? R Bardales; Mar?a J D?az-Enrich; Antonio Villamar?n
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't     Date:  2009-11-08
Journal Detail:
Title:  Journal of molecular histology     Volume:  40     ISSN:  1567-2387     ISO Abbreviation:  J. Mol. Histol.     Publication Date:  2009 Aug 
Date Detail:
Created Date:  2010-01-21     Completed Date:  2010-03-23     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  101193653     Medline TA:  J Mol Histol     Country:  Netherlands    
Other Details:
Languages:  eng     Pagination:  251-9     Citation Subset:  IM    
Affiliation:
Departamento de Bioqu?mica e Biolox?a Molecular, Universidade de Santiago de Compostela, Lugo, Spain.
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MeSH Terms
Descriptor/Qualifier:
Animals
Connective Tissue / enzymology
Cyclic AMP-Dependent Protein Kinases / metabolism*
Epithelium / enzymology
Female
Hemolymph / enzymology
Isoenzymes
Male
Mytilus / enzymology*
Ovarian Follicle / cytology,  enzymology
Spermatozoa / cytology,  enzymology
Chemical
Reg. No./Substance:
0/Isoenzymes; EC 2.7.11.11/Cyclic AMP-Dependent Protein Kinases

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