| Differential diagnosis of acute viral hepatitis using rapid, fully automated immunoassays. | |
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MedLine Citation:
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PMID: 1880494 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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We report the development of three rapid, fully automated immunoassays allowing the differential diagnosis of acute viral hepatitis. These assays detect HBsAg, IgM antibody to hepatitis B core antigen (IgM anti-HBc) and IgM antibody to hepatitis A virus (IgM anti-HAV) using the IMx instrument system. All IMx assays were run in less than 45 minutes and all steps were fully automated including specimen dilution steps. Specimens from blood donors, diagnostic and hospital patients, and individuals with a variety of infectious and immune diseases were tested for IgM anti-HAV (n = 1473) or for IgM anti-HBc (n = 1606) or for HBsAg (n = 9700) by the IMx and commercially available EIA and RIA. Each IMx assay showed 99.8% agreement with current EIA. Reproducibility in all hepatitis IMx assays was significantly better than that observed with manual or semiautomated assays; within-run and between-run % CV ranged from 2.2 to 4.8 and 3.5 to 10.3 respectively. In 29 acute hepatitis B patients studied, HBsAg and IgM anti-HBc were detected in the first available patient bleed collected from 0 to 4 week from the onset of symptoms. IgM anti-HBc persisted at reactive levels in the IMx assay for 1 to 24 weeks (mean 12.1 +/- 5.3 weeks) after the patient presented with symptoms. In individuals exposed to hepatitis A, IgM anti-HAV was detectable by IMx by 40 days post exposure (average 33.5 days) and IgM had declined to unreactive levels in IMx for all patients by from 3 to 6 months post exposure. These data demonstrate the use of these rapid IMx assays for differentiation of acute hepatitis A and B. |
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Authors:
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K Eble; J Clemens; C Krenc; M Rynning; J Stojak; J Stuckmann; P Hutten; L Nelson; L DuCharme; S Hojvat |
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Publication Detail:
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Type: Journal Article |
Journal Detail:
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Title: Journal of medical virology Volume: 33 ISSN: 0146-6615 ISO Abbreviation: J. Med. Virol. Publication Date: 1991 Mar |
Date Detail:
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Created Date: 1991-09-27 Completed Date: 1991-09-27 Revised Date: 2004-11-17 |
Medline Journal Info:
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Nlm Unique ID: 7705876 Medline TA: J Med Virol Country: UNITED STATES |
Other Details:
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Languages: eng Pagination: 139-50 Citation Subset: IM |
Affiliation:
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Abbott Laboratories, Abbott Park, Illinois 60064. |
Export Citation:
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APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
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Acute Disease Autoanalysis Diagnosis, Differential Hepatitis A / diagnosis* Hepatitis B / diagnosis* Hepatitis B Antibodies / analysis Hepatitis B Core Antigens / immunology Hepatitis B Surface Antigens / analysis Humans Immunoenzyme Techniques / instrumentation* Immunoglobulin G / immunology Reproducibility of Results Sensitivity and Specificity |
| Chemical | |
Reg. No./Substance:
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0/Hepatitis B Antibodies; 0/Hepatitis B Core Antigens; 0/Hepatitis B Surface Antigens; 0/Immunoglobulin G |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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