| Differential development of plasmacytoid dendritic cells in Th1- and Th2-like cytokine milieus. | |
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MedLine Citation:
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PMID: 21039603 Owner: NLM Status: In-Data-Review |
Abstract/OtherAbstract:
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To cite this article: Bratke K, Klein C, Kuepper M, Lommatzsch M, Christian Virchow J. Differential development of plasmacytoid dendritic cells in Th1- and Th2-like cytokine milieus. Allergy 2011; 66: 386-395. ABSTRACT: Background: Plasmacytoid dendritic cells (pDCs) infiltrate sites of Th1- and Th2-dominant inflammation and many studies have been performed to analyse their role in these immune responses. In contrast, much less is known about the effects of a Th1 or Th2 cytokine milieu on pDC function. Therefore, we investigated the impact of Th1- and Th2-like conditions during the development of pDCs on their antigen expression and function. Methods: PDCs were matured in vitro by the addition of IL-3 under Th1- or Th2-like conditions. Antigen expression and TLR7-ligand-induced cytokine secretion was analysed by flow cytometry and ELISA. Furthermore, the CD4(+) T-cell polarizing capacity of pDCs was determined as well as their potential to induce CD4(+) T-cell proliferation. Results: PDCs matured under Th1-like conditions showed a higher expression of antigens involved in T-cell co-stimulation and antigen presentation like CD40, CD80, CD83 and HLA-DR as well as a higher secretion of IL-6 and IFN-α in response to TLR7-ligation compared to Th2-pDCs. Furthermore, Th1-pDCs induced a significantly higher CD4(+) T-cell proliferation and primed a higher percentage of CD4(+) T cells to express IFN-γ and IL-2 after TLR7-ligation compared to Th2-pDCs. In contrast, Th2-pDCs were characterized by a significant upregulation of BDCA-3 and IL-4 expression following TLR7-ligation. Conclusion: This study is the first to demonstrate the crucial impact of a surrounding cytokine environment on the development of pDC function including antigen expression. Based on these findings, it can be speculated that antiviral/bacterial pDC functions could be impaired during acute allergic conditions. |
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Authors:
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K Bratke; C Klein; M Kuepper; M Lommatzsch; J Christian Virchow |
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Publication Detail:
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Type: Journal Article Date: 2010-10-29 |
Journal Detail:
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Title: Allergy Volume: 66 ISSN: 1398-9995 ISO Abbreviation: Allergy Publication Date: 2011 Mar |
Date Detail:
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Created Date: 2011-02-02 Completed Date: - Revised Date: - |
Medline Journal Info:
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Nlm Unique ID: 7804028 Medline TA: Allergy Country: Denmark |
Other Details:
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Languages: eng Pagination: 386-95 Citation Subset: IM |
Copyright Information:
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© 2010 John Wiley & Sons A/S. |
Affiliation:
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Department of Pneumology, University of Rostock, Rostock, Germany. |
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From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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