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Differential dendritic targeting of AMPA receptor subunit mrnas in adult rat hippocampal principal neurons and interneurons.
MedLine Citation:
PMID:  23296627     Owner:  NLM     Status:  Publisher    
Abstract/OtherAbstract:
In hippocampal neurons, AMPA receptors (AMPARs) mediate fast excitatory postsynaptic responses at glutamatergic synapses, and are involved in various forms of synaptic plasticity. Dendritic local protein synthesis of selected AMPAR subunit mRNAs is considered an additional mechanism to independently and rapidly control the strength of individual synapses. We have used fluorescent in situ hybridization and immunocytochemistry to analyse the localization of AMPAR subunit (GluA1-4) mRNAs and their relationship with the translation machinery in principal cells and interneurons of the adult rat hippocampus. The mRNAs encoding all four AMPAR subunits were detected in the somata and dendrites of CA3 and CA1 pyramidal cells and those of six classes of CA1 GABAergic interneurons. GluA1-4 subunit mRNAs were highly localized to the apical dendrites of pyramidal cells, while in interneurons they were present in multiple dendrites. In contrast, in dentate gyrus, GluA1-4 subunit mRNAs were virtually restricted to the somata and absent from the dendrites of granule cells. These different regional and cell type-specific labeling patterns also correlated with the localization of markers for components of the protein synthesis machinery. Our results support the local translation of GluA1-4 mRNAs in dendrites of hippocampal pyramidal cells and CA1 interneurons but not in granule cells of the dendate gyrus. Furthermore, the regional and cell type-specific differences we observed suggest that each cell type uses distinct ways of regulating the local translation of AMPAR subunits. J. Comp. Neurol., 2013. © 2013 Wiley Periodicals, Inc.
Authors:
David J Cox; Claudia Racca
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Publication Detail:
Type:  JOURNAL ARTICLE     Date:  2013-1-8
Journal Detail:
Title:  The Journal of comparative neurology     Volume:  -     ISSN:  1096-9861     ISO Abbreviation:  J. Comp. Neurol.     Publication Date:  2013 Jan 
Date Detail:
Created Date:  2013-1-8     Completed Date:  -     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  0406041     Medline TA:  J Comp Neurol     Country:  -    
Other Details:
Languages:  ENG     Pagination:  -     Citation Subset:  -    
Copyright Information:
Copyright © 2013 Wiley Periodicals, Inc.
Affiliation:
Institute of Neuroscience, Medical School, Newcastle University, Framlington Place, Newcastle-upon-Tyne, NE2 4HH, United Kingdom.
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