| Differential availability/processing of decorin precursor in arterial and venous smooth muscle cells. | |
| | |
MedLine Citation:
|
PMID: 16928198 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
|
The existence of specific differentiation markers for arterial smooth muscle (SM) cells is still a matter of debate. A clone named MM1 was isolated from a library of monoclonal antibodies to adult porcine aorta, which in vivo binds to arterial but not venous SM cells, except for the pulmonary vein. MM1 immunoreactivity in Western blotting involved bands in the range of M(r) 33-226 kDa, in both arterial and venous SM tissues. However, immunoprecipitation experiments revealed that MM1 bound to a 100-kDa polypeptide that was present only in the arterial SM extract. By mass spectrometry analysis of tryptic digests from MM1-positive 130- and 120-kDa polypeptides of aorta SM extract, the antigen recognized by the antibody was identified as a decorin precursor. Using a crude decorin preparation from this tissue MM1 reacted strongly with the 33-kDa polypeptide and this pattern did not change after chondroitinase ABC treatment. In vitro, decorin immunoreactivity was found in secreted grainy material produced by confluent arterial SM cells, although lesser amounts were also seen in venous SM cells. Western blotting of extracts from these cultures showed the presence of the 33-kDa band but not of the high-molecular-weight components, except for the 100-kDa monomer. The 100/33-kDa combination was more abundant in arterial SM cells than in the venous counterpart. In the early phase of neointima formation, induced by endothelial injury of the carotid artery or vein-to-artery transposition, the decorin precursor was not expressed, but it was up-regulated in the SM cells of the media underlying the neointima in both models. Collectively, these data suggest a different processing/utilization of the 100-kDa monomer of proteoglycan decorin in arterial and venous SM cells, which is abolished after vein injury. |
| | |
Authors:
|
Rafaella Franch; Angela Chiavegato; Maddalena Maraschin; Serena Candeo; Simonetta Ausoni; Antonello Villa; Gino Gerosa; Lisa Gasparotto; Pierpaolo Parnigotto; Saverio Sartore |
Related Documents
:
|
20121538 - From monster to twin reversed arterial perfusion: a history of acardiac twins. 3510328 - Distribution of venous valvular incompetence in patients with the postphlebitic syndrome. 7802188 - The need for quality control in measurement of mixed venous oxygen saturation. 19139028 - Development of novel drug-eluting biodegradable nano-fiber for prevention of postoperat... 868898 - Echocardiography in valvular vegetations. 8567558 - Secretory leukoprotease inhibitor attenuates lung injury induced by continuous air embo... |
Publication Detail:
|
Type: Comparative Study; Journal Article; Research Support, Non-U.S. Gov't |
Journal Detail:
|
Title: Journal of anatomy Volume: 209 ISSN: 0021-8782 ISO Abbreviation: J. Anat. Publication Date: 2006 Sep |
Date Detail:
|
Created Date: 2006-08-24 Completed Date: 2007-01-18 Revised Date: 2009-11-18 |
Medline Journal Info:
|
Nlm Unique ID: 0137162 Medline TA: J Anat Country: England |
Other Details:
|
Languages: eng Pagination: 271-87 Citation Subset: IM |
Affiliation:
|
Department of Biomedical Sciences, University of Padua, Padua, Italy. |
Export Citation:
|
APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
|
Animals Animals, Newborn Antibodies, Monoclonal / immunology* Antibody Specificity Antigen-Antibody Reactions Aorta Blood Vessel Prosthesis Implantation Blotting, Western Cells, Cultured Coronary Vessels Extracellular Matrix Proteins / immunology* Female Humans Hybridomas Immunoprecipitation Jugular Veins Mice Mice, Inbred BALB C Microscopy, Confocal Muscle, Smooth, Vascular* Myocytes, Smooth Muscle / immunology* Proteoglycans / immunology* Pulmonary Veins Swine |
| Chemical | |
Reg. No./Substance:
|
0/Antibodies, Monoclonal; 0/Extracellular Matrix Proteins; 0/Proteoglycans; 0/decorin |
| Comments/Corrections | |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
Previous Document: Degradation of the hexose transporter Hxt5p in Saccharomyces cerevisiae.
Next Document: Intra- and intermuscular variation in human quadriceps femoris architecture assessed in vivo.