Document Detail


Differential availability/processing of decorin precursor in arterial and venous smooth muscle cells.
MedLine Citation:
PMID:  16928198     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
The existence of specific differentiation markers for arterial smooth muscle (SM) cells is still a matter of debate. A clone named MM1 was isolated from a library of monoclonal antibodies to adult porcine aorta, which in vivo binds to arterial but not venous SM cells, except for the pulmonary vein. MM1 immunoreactivity in Western blotting involved bands in the range of M(r) 33-226 kDa, in both arterial and venous SM tissues. However, immunoprecipitation experiments revealed that MM1 bound to a 100-kDa polypeptide that was present only in the arterial SM extract. By mass spectrometry analysis of tryptic digests from MM1-positive 130- and 120-kDa polypeptides of aorta SM extract, the antigen recognized by the antibody was identified as a decorin precursor. Using a crude decorin preparation from this tissue MM1 reacted strongly with the 33-kDa polypeptide and this pattern did not change after chondroitinase ABC treatment. In vitro, decorin immunoreactivity was found in secreted grainy material produced by confluent arterial SM cells, although lesser amounts were also seen in venous SM cells. Western blotting of extracts from these cultures showed the presence of the 33-kDa band but not of the high-molecular-weight components, except for the 100-kDa monomer. The 100/33-kDa combination was more abundant in arterial SM cells than in the venous counterpart. In the early phase of neointima formation, induced by endothelial injury of the carotid artery or vein-to-artery transposition, the decorin precursor was not expressed, but it was up-regulated in the SM cells of the media underlying the neointima in both models. Collectively, these data suggest a different processing/utilization of the 100-kDa monomer of proteoglycan decorin in arterial and venous SM cells, which is abolished after vein injury.
Authors:
Rafaella Franch; Angela Chiavegato; Maddalena Maraschin; Serena Candeo; Simonetta Ausoni; Antonello Villa; Gino Gerosa; Lisa Gasparotto; Pierpaolo Parnigotto; Saverio Sartore
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Publication Detail:
Type:  Comparative Study; Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Journal of anatomy     Volume:  209     ISSN:  0021-8782     ISO Abbreviation:  J. Anat.     Publication Date:  2006 Sep 
Date Detail:
Created Date:  2006-08-24     Completed Date:  2007-01-18     Revised Date:  2013-06-07    
Medline Journal Info:
Nlm Unique ID:  0137162     Medline TA:  J Anat     Country:  England    
Other Details:
Languages:  eng     Pagination:  271-87     Citation Subset:  IM    
Affiliation:
Department of Biomedical Sciences, University of Padua, Padua, Italy.
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MeSH Terms
Descriptor/Qualifier:
Animals
Animals, Newborn
Antibodies, Monoclonal / immunology*
Antibody Specificity
Antigen-Antibody Reactions
Aorta
Blood Vessel Prosthesis Implantation
Blotting, Western
Cells, Cultured
Coronary Vessels
Decorin
Extracellular Matrix Proteins / immunology*
Female
Humans
Hybridomas
Immunoprecipitation
Jugular Veins
Mice
Mice, Inbred BALB C
Microscopy, Confocal
Muscle, Smooth, Vascular*
Myocytes, Smooth Muscle / immunology*
Proteoglycans / immunology*
Pulmonary Veins
Swine
Chemical
Reg. No./Substance:
0/Antibodies, Monoclonal; 0/DCN protein, human; 0/Dcn protein, mouse; 0/Decorin; 0/Extracellular Matrix Proteins; 0/Proteoglycans
Comments/Corrections

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