| Differential characterization of holocentric chromosomes in triatomines (Heteroptera, Triatominae) using different staining techniques and fluorescent in situ hybridization. | |
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MedLine Citation:
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PMID: 18050092 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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A comparative study of holocentric chromosomes in the triatomine species Panstrongylus megistus, Rhodnius pallescens and Triatoma infestans was carried out in order to characterize heterochromatin, rDNA active sites and nucleolar proteins. Cytological preparations of seminiferous tubules were stained by silver impregnation, C banding, fluorochromes cma3/da and dapi/da, and fluorescent in situ hybridization (FISH) with Drosophila melanogaster 28S rDNA probe. Our results showed interesting aspects of the organization of chromatin and chromosomes in the meiotic cells of these insects. In R. pallescens, sex chromosomes (X, Y) were distinct from autosomes, when submitted to silver impregnation, C banding, CMA3 staining, and FISH, confirming that these chromosomes bear nucleolar organizer regions (NORs). In P. megistus, two of the three sex chromosomes were CMA3/DAPI-; at early meiotic prophase and at diakinesis, silver impregnation corresponded with FISH signals, indicating that in this species, two chromosomes (probably a sex chromosome and an autosome) bear NORs. In T. infestans, silver nitrate and FISH also stained corresponding areas on meiotic chromosomes. Our data suggest that in triatomines, in general, the number and location of NORs are species-specific. These regions may be considered important chromosome markers for comparative studies to improve the understanding of evolutionary mechanisms in these hematophagous insects. |
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Authors:
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A Morielle-Souza; M T V Azeredo-Oliveira |
Publication Detail:
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Type: Journal Article; Research Support, Non-U.S. Gov't Date: 2007-09-30 |
Journal Detail:
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Title: Genetics and molecular research : GMR Volume: 6 ISSN: 1676-5680 ISO Abbreviation: Genet. Mol. Res. Publication Date: 2007 |
Date Detail:
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Created Date: 2007-11-30 Completed Date: 2008-01-14 Revised Date: - |
Medline Journal Info:
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Nlm Unique ID: 101169387 Medline TA: Genet Mol Res Country: Brazil |
Other Details:
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Languages: eng Pagination: 713-20 Citation Subset: IM |
Affiliation:
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Departamento de Biologia, Instituto de Biociências, Letras e Ciências Exatas, IBILCE-UNESP, São José do Rio Preto, SP, Brasil. |
Export Citation:
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| MeSH Terms | |
Descriptor/Qualifier:
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Animals Chromosomes / metabolism* In Situ Hybridization, Fluorescence / methods* Male Panstrongylus / genetics Rhodnius / genetics Seminiferous Tubules / cytology Staining and Labeling / methods* Triatoma / genetics Triatominae / genetics* |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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