| Differential carbon source utilization by Campylobacter jejuni 11168 in response to growth temperature variation. | |
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MedLine Citation:
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PMID: 20035808 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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Campylobacter spp. readily colonize the intestinal tracts of both human and avian species. While most often commensal organisms in birds, campylobacters remain the leading cause of bacterial gastroenteritis in humans. The association of campylobacters with poultry is well established as a primary route for human exposure. The difference in normal core body temperature between chickens (42 degrees C) and humans (37 degrees C) has been suggested to trigger potential colonization or virulence factors and investigators have demonstrated differential gene expression at the two temperatures. Campylobacter spp. exhibit unique nutritional requirements and have been thought to only utilize amino acids and Kreb cycle intermediates as carbon sources for growth. We evaluated the ability of the genome-sequenced strain of Campylobacter jejuni 11168 (GS) to oxidize 190 different substrates as sole carbon sources at 37 degrees C and 42 degrees C using phenotype microarray (PM) technology. Results indicate that the expected amino acids, l-serine, l-aspartic acid, l-asparagine, and l-glutamic acid were utilized in addition to a number of organic acids. In general, oxidation of the substrates was greater at 42 degrees C than at 37 degrees C with a few exceptions. By employing the PM method, we observed a number of potential false-positive reactions for substrates including the triose, dihydroxyacetone; and the pentose sugars, d-xylose, d-ribose, l-lyxose, and d- and l-arabinose. The presence of genes possibly responsible for utilization of pentose sugars is supported by the genomic sequence data, but actual utilization as sole carbon sources for active respiration has not been observed. A better understanding of the metabolic pathways and nutritional requirements of campylobacters could lead to improvements in culture media for detection and isolation of the pathogen and to future intervention methods to reduce human exposure. |
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Authors:
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J E Line; K L Hiett; J Guard-Bouldin; B S Seal |
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Publication Detail:
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Type: Journal Article; Research Support, U.S. Gov't, Non-P.H.S. Date: 2009-12-24 |
Journal Detail:
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Title: Journal of microbiological methods Volume: 80 ISSN: 1872-8359 ISO Abbreviation: J. Microbiol. Methods Publication Date: 2010 Feb |
Date Detail:
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Created Date: 2010-02-01 Completed Date: 2010-03-30 Revised Date: - |
Medline Journal Info:
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Nlm Unique ID: 8306883 Medline TA: J Microbiol Methods Country: Netherlands |
Other Details:
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Languages: eng Pagination: 198-202 Citation Subset: IM |
Copyright Information:
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Published by Elsevier B.V. |
Affiliation:
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USDA ARS, Russell Research Center, P.O. Box 5677, Athens, GA 30604, USA. eric.line@ars.usda.gov |
Export Citation:
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APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
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Adaptation, Physiological Amino Acids / metabolism Animals Bacterial Typing Techniques / methods Campylobacter jejuni / metabolism*, radiation effects* Carbon / metabolism* Carboxylic Acids / metabolism Humans Phenotype Temperature* |
| Chemical | |
Reg. No./Substance:
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0/Amino Acids; 0/Carboxylic Acids; 7440-44-0/Carbon |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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