Document Detail


Differential blockage of charge movement components in frog cut twitch fibres by nifedipine.
MedLine Citation:
PMID:  1822564     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
1. The effect of nifedipine on charge movement was studied in cut twitch fibres of Rana temporaria with a double Vaseline-gap voltage-clamp technique. The steady-state charge-voltage (Q-V) plot, in the absence or presence of nifedipine, was separated into Q beta and Q gamma components by fitting with a sum of two Boltzmann distribution functions. 2. When a fibre was held at -90 mV, low concentrations (around 20 nM) of nifedipine suppressed a large fraction of Q beta than Q gamma. Higher concentrations of nifedipine suppressed Q gamma more effectively than Q beta, but even 2 microM-nifedipine did not suppress Q beta and Q gamma completely. Ten micromolar was required for complete suppression of Q gamma. Nifedipine thus suppressed Q beta and Q gamma with different dose dependencies. 3. When the holding potential was changed to -70 mV, some Q beta and Q gamma were inactivated. Low concentrations (around 20 nM) of nifedipine still suppressed a larger fraction of the mobile Q beta than the mobile Q gamma. Higher concentrations of nifedipine also suppressed Q gamma more effectively than Q beta, but 2 microM-nifedipine was sufficient to suppress Q gamma completely. Hence, at this slightly depolarized holding potential, nifedipine also suppressed Q beta and Q gamma with different dose dependencies. 4. A portion of Q beta appeared to be resistant to the action of nifedipine. At -70 mV, the blockage of the nifedipine-sensitive portion of Q beta appeared to saturate at 2 microM of the drug. At -90 mV, the nifedipine-resistant portion of Q beta was more difficult to identify, because the blockage of the nifedipine-sensitive portion of Q beta did not saturate at 2 microM. 5. Based on double-reciprocal plots for the dose-response relationships, the half-blocking concentration of nifedipine for Q beta was found to be 14-19 nM at -90 mV and less than 13 nM at -70 mV, whereas that for Q gamma was approximately 1.6 microM at -90 mV and 120 nM at -70 mV. Thus, nifedipine suppressed Q gamma in a voltage-dependent manner, but its suppression of Q beta was much less voltage dependent. 6. It was demonstrated that the enhancement in the blockage of charge movement by maintained depolarization could not be achieved by depolarizing pulses lasting up to hundreds of milliseconds. 7. The difference in the half-blocking concentrations of nifedipine for Q beta and Q gamma implies that Q beta and Q gamma cannot be tightly coupled.
Authors:
W Chen; C S Hui
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Publication Detail:
Type:  In Vitro; Journal Article; Research Support, Non-U.S. Gov't; Research Support, U.S. Gov't, P.H.S.    
Journal Detail:
Title:  The Journal of physiology     Volume:  444     ISSN:  0022-3751     ISO Abbreviation:  J. Physiol. (Lond.)     Publication Date:  1991 Dec 
Date Detail:
Created Date:  1992-08-31     Completed Date:  1992-08-31     Revised Date:  2009-11-18    
Medline Journal Info:
Nlm Unique ID:  0266262     Medline TA:  J Physiol     Country:  ENGLAND    
Other Details:
Languages:  eng     Pagination:  579-603     Citation Subset:  IM    
Affiliation:
Department of Physiology and Biophysics, Indiana University Medical Center, Indianapolis 46223.
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MeSH Terms
Descriptor/Qualifier:
Animals
Membrane Potentials
Muscles / drug effects*,  physiology
Nifedipine / pharmacology*
Rana temporaria
Grant Support
ID/Acronym/Agency:
NS21955/NS/NINDS NIH HHS
Chemical
Reg. No./Substance:
21829-25-4/Nifedipine
Comments/Corrections

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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