Document Detail

Differential binding of nuclear factors to the intron 1 sequences containing the transcriptional pause site correlates with c-myb expression.
MedLine Citation:
PMID:  2678098     Owner:  NLM     Status:  MEDLINE    
The molecular mechanisms that modulate c-myb mRNA levels in hematopoietic cells appear to involve premature termination of transcription in the first intron of the gene. We have examined the DNA-protein interactions within the first intron of the c-myb gene and identified a 1.0-kilobase region that could be responsible for its transcriptional regulation. Using the mobility-shift assay, we show a direct correlation between the extent of sequence-specific protein binding to intron 1 DNA fragments, and c-myb mRNA levels in different cell types. During dimethyl sulfoxide-induced differentiation of mouse erythroleukemic cells, there was a dramatic decrease in these nuclear factors that correlated with the decrease in the levels of c-myb mRNA. Nucleotide sequence analysis and DNase I footprinting revealed the presence of putative regulatory elements that are implicated in the binding of these nuclear factors. We propose that binding of nuclear factors to the site of transcriptional pause could play an important role in the regulation of c-myb transcription.
C D Reddy; E P Reddy
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't; Research Support, U.S. Gov't, P.H.S.    
Journal Detail:
Title:  Proceedings of the National Academy of Sciences of the United States of America     Volume:  86     ISSN:  0027-8424     ISO Abbreviation:  Proc. Natl. Acad. Sci. U.S.A.     Publication Date:  1989 Oct 
Date Detail:
Created Date:  1989-11-09     Completed Date:  1989-11-09     Revised Date:  2009-11-18    
Medline Journal Info:
Nlm Unique ID:  7505876     Medline TA:  Proc Natl Acad Sci U S A     Country:  UNITED STATES    
Other Details:
Languages:  eng     Pagination:  7326-30     Citation Subset:  IM    
Wistar Institute, Philadelphia, PA 19104.
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MeSH Terms
Base Sequence
Cell Line
Cell Nucleus / metabolism
DNA Probes
Leukemia, Erythroblastic, Acute
Molecular Sequence Data
Proto-Oncogene Proteins / genetics*
Proto-Oncogene Proteins c-myb
RNA, Messenger / genetics
Restriction Mapping
Transcription, Genetic*
Grant Support
Reg. No./Substance:
0/DNA Probes; 0/Proto-Oncogene Proteins; 0/Proto-Oncogene Proteins c-myb; 0/RNA, Messenger

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