| Differential appearance of extensively phosphorylated forms of the high molecular weight neurofilament protein in regions of mouse brain during postnatal development. | |
| | |
MedLine Citation:
|
PMID: 1898519 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
|
The appearance and accumulation of extensively phosphorylated forms of the high molecular weight neurofilament protein (H-phos) was studied in six regions of mouse brain during postnatal development by quantitative immunoblot analyses. H-phos (migrating at 200 kDa) was detected in brainstem, cerebellum, cortex and hippocampus as early as postnatal day 1. While NF-H levels increased dramatically during subsequent postnatal development in these regions, and reached levels similar to those observed in adult brain by postnatal day 14, quantitative differences were observed in both the rate and the extent of increase among individual regions. The most rapid accumulation of H-phos was observed in brainstem and cortex, where H-phos increased within the first postnatal week to levels comparable to those of adult brain. However, H-phos exhibited a slower developmental change in cerebellum, where the levels increased uniformly over the first two postnatal weeks. In hippocampus, the major increase in H-phos levels was delayed until the second postnatal week. In contrast to its early detection in the above regions, H-phos was not detected in immunoblot analyses of olfactory bulb or hypothalamus cytoskeletons at postnatal day 1, indicating that in these regions the accumulated levels of posttranslationally modified forms of this protein appeared relatively late. Furthermore, H-phos levels in hippocampus did not level off at postnatal day 14 and continued to increase until at least postnatal day 21. Immunoblot analyses of whole embryonic brain revealed the presence of H-phos as early as embryonic day 17, demonstrating that some mouse brain regions carry out extensive phosphorylation of NF-H during embryonic development.(ABSTRACT TRUNCATED AT 250 WORDS) |
| | |
Authors:
|
I Fischer; T B Shea |
Related Documents
:
|
21860219 - Integrated brain diversification along the early neuraxes. 15160389 - Alpha-dystroglycan interactions affect cerebellar granule neuron migration. 11313299 - Early-generated preplate neurons in the developing telencephalon: inward migration into... 9405699 - Postnatal mouse subventricular zone neuronal precursors can migrate and differentiate w... 20146799 - Signaling involved in neurite outgrowth of postnatally born subventricular zone neurons... 19782409 - Making a neuron: cdk5 in embryonic and adult neurogenesis. 1697809 - The time course of retrograde transsynaptic transport of tetanus toxin fragment c in th... 15829609 - Neuronal representations of stimuli in the mouth: the primate insular taste cortex, orb... 728969 - Segmental and interganglionic projections from primary fibres of insect mechanoreceptors. |
Publication Detail:
|
Type: Journal Article; Research Support, U.S. Gov't, Non-P.H.S.; Research Support, U.S. Gov't, P.H.S. |
Journal Detail:
|
Title: Journal of neuroimmunology Volume: 31 ISSN: 0165-5728 ISO Abbreviation: J. Neuroimmunol. Publication Date: 1991 Jan |
Date Detail:
|
Created Date: 1991-01-24 Completed Date: 1991-01-24 Revised Date: 2007-11-14 |
Medline Journal Info:
|
Nlm Unique ID: 8109498 Medline TA: J Neuroimmunol Country: NETHERLANDS |
Other Details:
|
Languages: eng Pagination: 73-81 Citation Subset: IM |
Affiliation:
|
Department of Biochemistry, E.K. Shriver Center, Waltham, MA 02254. |
Export Citation:
|
APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
|
Animals Brain / growth & development* Brain Chemistry* Intermediate Filament Proteins / analysis*, genetics Mice Molecular Weight Neurofilament Proteins Phosphorylation RNA, Messenger / analysis |
| Grant Support | |
ID/Acronym/Agency:
|
NS24725/NS/NINDS NIH HHS |
| Chemical | |
Reg. No./Substance:
|
0/Intermediate Filament Proteins; 0/Neurofilament Proteins; 0/RNA, Messenger |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
Previous Document: Tetraethylammonium, a K+ channel blocker, inhibits interferon-gamma-induced major histocompatibility...
Next Document: Endoscopic injection of glutaraldehyde cross-linked bovine dermal collagen for correction of vesicou...