| Differential apoptotic staining of mammalian blastocysts based on double immunofluorescent CDX2 and active caspase-3 staining. | |
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MedLine Citation:
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PMID: 21684250 Owner: NLM Status: Publisher |
Abstract/OtherAbstract:
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Several approaches have been described for differential staining of blastocysts, but these methods are often time-consuming and unreliable. Here we describe a method for simultaneous differential staining and detection of apoptosis. The differential staining is based on the transcription factor CDX2 which is localized in the nucleus of trophectoderm (TE) cells but absent in the inner cell mass (ICM). Apoptosis is detected by staining of active caspase-3, a key player in several apoptotic pathways. This new approach represents a robust method for quantifying simultaneously ICM/TE ratio and apoptotic cell ratio in bovine, murine, porcine, and human blastocysts. |
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Authors:
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Eline Wydooghe; Leen Vandaele; Josine Beek; Herman Favoreel; Björn Heindryckx; Petra De Sutter; Ann Van Soom |
Publication Detail:
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Type: JOURNAL ARTICLE Date: 2011-5-27 |
Journal Detail:
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Title: Analytical biochemistry Volume: - ISSN: 1096-0309 ISO Abbreviation: - Publication Date: 2011 May |
Date Detail:
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Created Date: 2011-6-20 Completed Date: - Revised Date: - |
Medline Journal Info:
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Nlm Unique ID: 0370535 Medline TA: Anal Biochem Country: - |
Other Details:
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Languages: ENG Pagination: - Citation Subset: - |
Copyright Information:
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Copyright © 2011 Elsevier Inc. All rights reserved. |
Affiliation:
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Faculty of Veterinary Medicine, Ghent University, B-9820 Merelbeke, Belgium. |
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From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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