Document Detail


Differential alveolar epithelial injury and protein expression in pneumococcal pneumonia.
MedLine Citation:
PMID:  22563685     Owner:  NLM     Status:  In-Data-Review    
Abstract/OtherAbstract:
ABSTRACT The integrity of the alveolar epithelium is a key factor in the outcome of acute lung injury. Here, we investigate alveolar epithelial injury and the expression of epithelial-selective markers in Streptococcus pneumoniae-induced acute lung injury. S. pneumoniae was instilled into rat lungs and alveolar type I (RTI(40)/podoplanin, MMC6 antigen) and alveolar type II (MMC4 antigen, surfactant protein D, pro-surfactant protein C, RTII(70)) cell markers were quantified in lavage fluid and lung tissue at 24 and 72 hours. The alveolar epithelium was also examined using electron, confocal, and light microscopy. S. pneumoniae induced an acute inflammatory response as assessed by increased total protein, SP-D, and neutrophils in lavage fluid. Biochemical and morphological studies demonstrated morphologic injury to type II cells but not type I cells. In particular, the expression of RTI(40)/podoplanin was dramatically reduced, on the surface of type I cells, in the absence of morphologic injury. These data demonstrate that type II cell damage can occur in the absence of type I cell injury without affecting the ability of the lung to return to a normal morphology. These data also demonstrate that RTI(40)/podoplanin is not a type I cell phenotypic marker in experimental acute lung injury caused by S. pneumoniae. Given that RTI(40)/podoplanin is an endogenous ligand for the C-type lectin receptor and this receptor plays a role in platelet aggregation and neutrophil activation, we hypothesize that the reduction of RTI(40)/podoplanin on type I cells might be important for the regulation of platelet and/or neutrophil function in experimental acute lung injury.
Authors:
Christine Tyrrell; Stuart R McKechnie; Michael F Beers; Tim J Mitchell; Mary C McElroy
Related Documents :
15306645 - Disrupted spermine homeostasis: a novel mechanism in polyglutamine-mediated aggregation...
12697895 - Stable and controllable rna interference: investigating the physiological function of g...
15782175 - Rnai living-cell microarrays for loss-of-function screens in drosophila melanogaster ce...
20018615 - Rna interference screen in primary human t cells reveals flt3 as a modulator of il-10 l...
21181095 - Na+/k+-atpase α3 mediates sensitivity of hepatocellular carcinoma cells to bufalin.
22860235 - Notch receptor inhibition reveals the importance of cyclin d1 and wnt signaling in inva...
Publication Detail:
Type:  Journal Article    
Journal Detail:
Title:  Experimental lung research     Volume:  38     ISSN:  1521-0499     ISO Abbreviation:  Exp. Lung Res.     Publication Date:  2012 Jun 
Date Detail:
Created Date:  2012-05-08     Completed Date:  -     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  8004944     Medline TA:  Exp Lung Res     Country:  England    
Other Details:
Languages:  eng     Pagination:  266-76     Citation Subset:  IM    
Affiliation:
1MRC Centre for Inflammation Research, Queen's Medical Research Institute, University of Edinburgh, Edinburgh, Scotland, UK.
Export Citation:
APA/MLA Format     Download EndNote     Download BibTex
MeSH Terms
Descriptor/Qualifier:

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


Previous Document:  ?-Nicotinamide adenine dinucleotide attenuates lipopolysaccharide-induced inflammatory effects in a ...
Next Document:  Hypoxia impedes hypertrophic chondrogenesis of human multipotent stromal cells.