| Differential alterations in metabolic pattern of the spliceosomal uridylic acid-rich small nuclear RNAs (UsnRNAs) during malignant transformation of 20-methylcholanthrene-induced mouse CNCI-PM-20 embryonic fibroblasts. | |
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MedLine Citation:
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PMID: 19496104 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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Differential alterations of the spliceosomal Uridylic acid rich small nuclear RNAs (UsnRNAs) (U1, U2, U4, U5, and U6) are reported to be associated with cellular proliferation and development, but definitive information is scarce and also elusive. An attempt is made in this study to analyze the metabolic patterns of major spliceosomal UsnRNAs, during tumor development, in an in vitro carcinogenesis model of 20-methylcholanthrene (MCA)-transformed Swiss Mouse Embryonic Fibroblast (MEF), designated as CNCI-PM-20. MEF cells, after treatment with 20-MCA, progressed through a sequence of passages with distinct and heritable changes, finally becoming neoplastic at passage-42 (P42). A differential expression pattern of major UsnRNAs was observed during this process. The abundance of U1 was 20% below control (P1) at passage-20 (P20), followed by a gradual increase up until P42 (approximately 12% above the P1 value). The abundance of U2 was more or less constant during the cellular transformation. U4 showed a trend of increase, with above 30% abundance than control at P20, followed by a significant increase at P36 and P42 (1.5- and 2-fold, respectively, P-value <0.01). U5 also followed an identical pattern, with an increase of 70% compared to control (P-value <0.05) at P42. Interestingly, U6 gradually decreased from P20 onwards up until P42, with 22% at P20 and 67% at P42 (P-value <0.01). An overall significant quantitative alteration in abundance of U4, U5, and U6, observed in our study, contributes to the understanding of the fact that, the metabolism of major spliceosomal UsnRNAs is differentially regulated during the process of neoplastic transformation. |
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Authors:
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Sudeshna Mukherjee; Sugata Manna; Pratima Mukherjee; Chinmay K Panda |
Publication Detail:
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Type: Journal Article; Research Support, Non-U.S. Gov't |
Journal Detail:
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Title: Molecular carcinogenesis Volume: 48 ISSN: 1098-2744 ISO Abbreviation: Mol. Carcinog. Publication Date: 2009 Sep |
Date Detail:
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Created Date: 2009-09-01 Completed Date: 2009-09-16 Revised Date: - |
Medline Journal Info:
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Nlm Unique ID: 8811105 Medline TA: Mol Carcinog Country: United States |
Other Details:
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Languages: eng Pagination: 773-8 Citation Subset: IM |
Affiliation:
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Department of Oncogene Regulation, Chittaranjan National Cancer Institute, Kolkata 700026, India. |
Export Citation:
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| MeSH Terms | |
Descriptor/Qualifier:
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Animals Cell Transformation, Neoplastic / drug effects*, genetics Cells, Cultured Embryo, Mammalian / cytology Fibroblasts / cytology, drug effects*, metabolism Gene Expression / drug effects Gene Expression Profiling Methylcholanthrene / toxicity* Mice RNA, Small Nuclear / genetics*, metabolism Spliceosomes / drug effects, genetics, metabolism Uridine Monophosphate / metabolism |
| Chemical | |
Reg. No./Substance:
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0/RNA, Small Nuclear; 0/U1 small nuclear RNA; 0/U2 small nuclear RNA; 0/U4 small nuclear RNA; 0/U5 small nuclear RNA; 0/U6 small nuclear RNA; 56-49-5/Methylcholanthrene; 58-97-9/Uridine Monophosphate |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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