Document Detail


Differential alterations of ethanolamine and choline phosphoglyceride metabolism by clofibrate and retinoic acid in human fibroblasts are not mediated by phorbol ester-sensitive protein kinase C.
MedLine Citation:
PMID:  8827698     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
Peroxisomal proliferators and retinoids have been reported to interact to regulate lipid metabolism, particularly beta-oxidation of fatty acids. Based on postulated interactions of these agents at the levels of receptors and response elements, we examined whether interactions exist between the peroxisomal proliferator, clofibrate (CLF), and retinoic acid (RA) in modulation of phospholipid turnover in cultured human skin fibroblasts. Treatment of cultured cells with either 25 microM CLF or 1 microM RA alone decreased [14C]ethanolamine incorporation into ethanolamine phosphoglycerides (EPG) by 20-30%, and simultaneous exposure to both agents resulted in additive inhibition. By contrast, [3H]choline incorporation into phospholipid was stimulated 5-30% by incubation with either agent; when CLF and RA were administered together, the stimulatory effects were additive. Different types of pulse-chase studies examining effects on uptake, biosynthesis, and degradation of labelled phospholipids indicated stimulation of EPG degradation and inhibition of phosphatidylcholine degradation by CLF; no effect on catabolism of either phospholipid was observed with RA. Combinations of modifiers of protein kinase activity [4 beta-12-O-tetradecanoylphorbol-13-acetate (beta-TPA), 1-(5-isoquinolinesulfonyl)-2-methylpiperazine dihydrochloride, N-(2'-guanidinoethyl)-5-isoquinolinesulfonamide hydrochloride, bis-indolylmaleimide, staurosporine indicated that beta-TPA-responsive protein kinases were not involved. Accordingly, CLF and RA regulate biosynthesis and degradation of ethanolamine and choline phosphoglycerides in cultured skin fibroblasts by different mechanisms that do not involve classical protein kinase C (PKC) isoforms, even though turnover of phospholipids generating lipid activators of PKC occurs.
Authors:
S G Mandla; D M Byers; N D Ridgway; H W Cook
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Publication Detail:
Type:  Comparative Study; Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Lipids     Volume:  31     ISSN:  0024-4201     ISO Abbreviation:  Lipids     Publication Date:  1996 Jul 
Date Detail:
Created Date:  1996-12-17     Completed Date:  1996-12-17     Revised Date:  2007-11-15    
Medline Journal Info:
Nlm Unique ID:  0060450     Medline TA:  Lipids     Country:  UNITED STATES    
Other Details:
Languages:  eng     Pagination:  747-55     Citation Subset:  IM    
Affiliation:
Atlantic Research Centre, Department of Pediatrics, Dalhousie University, Halifax, Nova Scotia, Canada.
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MeSH Terms
Descriptor/Qualifier:
1-(5-Isoquinolinesulfonyl)-2-Methylpiperazine / pharmacology
Antilipemic Agents / pharmacology*
Carcinogens / pharmacology
Clofibrate / pharmacology*
Enzyme Inhibitors / pharmacology
Ethanolamine
Ethanolamines / metabolism*
Fibroblasts / drug effects,  metabolism,  secretion
Humans
Isoquinolines / pharmacology
Phosphatidylcholines / metabolism*
Phospholipids / metabolism
Protein Kinase C / antagonists & inhibitors,  drug effects,  metabolism*
Skin / cytology
Staurosporine / pharmacology
Sulfonamides*
Tetradecanoylphorbol Acetate / pharmacology
Time Factors
Tretinoin / pharmacology*
Chemical
Reg. No./Substance:
0/Antilipemic Agents; 0/Carcinogens; 0/Enzyme Inhibitors; 0/Ethanolamines; 0/Isoquinolines; 0/Phosphatidylcholines; 0/Phospholipids; 0/Sulfonamides; 141-43-5/Ethanolamine; 16561-29-8/Tetradecanoylphorbol Acetate; 302-79-4/Tretinoin; 62996-74-1/Staurosporine; 637-07-0/Clofibrate; 84477-87-2/1-(5-Isoquinolinesulfonyl)-2-Methylpiperazine; 91742-10-8/N-(2-guanidinoethyl)-5-isoquinolinesulfonamide; EC 2.7.11.13/Protein Kinase C

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