| Differential activations of PKC/PKA related to microvasculopathy in diabetic GK rats. | |
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MedLine Citation:
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PMID: 21989030 Owner: NLM Status: Publisher |
Abstract/OtherAbstract:
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Microvasculopathy is the most serious and predictable threat to the health of diabetic patients, which often results in end-stage renal disease, blindness, and limb amputations. Up to the present the underlying mechanisms have remained elusive. Here it was found that the differential activations of PKC/PKA were involved in diabetic microvasculopathy in diabetic GK rats. By real-time PCR, Western blot, immunohistochemistry and enzyme activity assay, the up-regulation of PKC was prominent in kidney, but it was not significant in liver and brain. The expression and activity of PKA were lowered in kidney but comparable in brain and liver during diabetic nephropathy. Furthermore the generation of reactive oxygen species, the production of nitric oxide and the expression of inducible nitric oxide synthase induced by advanced glycation end products were inhibited by PKC-β inhibitor LY333531 or a PKA agonist in rat glomerular microvascular endothelial cells. Finally albuminuria was significantly lowered by a PKA agonist and boosted by a PKA antagonist. It suggested that the differential activations of PKC/PKA related to microvasculopathy in diabetes and the activation of PKA may protect the diabetic microvasculopathy. |
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Authors:
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Hui Wang; Yong-Wei Jiang; Wen-Jian Zhang; Shi-Qing Xu; Hong-Lin Liu; Wen-Ying Yang; Jin-Ning Lou |
Publication Detail:
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Type: JOURNAL ARTICLE Date: 2011-10-11 |
Journal Detail:
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Title: American journal of physiology. Endocrinology and metabolism Volume: - ISSN: 1522-1555 ISO Abbreviation: - Publication Date: 2011 Oct |
Date Detail:
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Created Date: 2011-10-12 Completed Date: - Revised Date: - |
Medline Journal Info:
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Nlm Unique ID: 100901226 Medline TA: Am J Physiol Endocrinol Metab Country: - |
Other Details:
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Languages: ENG Pagination: - Citation Subset: - |
Affiliation:
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1Peking Union Medical College. |
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From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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