Document Detail

Differential activation of the volume-sensitive cation channel TRP12 (OTRPC4) and volume-regulated anion currents in HEK-293 cells.
MedLine Citation:
PMID:  11713648     Owner:  NLM     Status:  MEDLINE    
The detection of changes in volume and osmolality is an essential function in vertebrate cells. A novel member of the transient receptor potential (trp) family of ion channels, which is sensitive to changes in cell volume, has been described recently. Heterologous expression of TRP12 in HEK cells resulted in the appearance of a swelling-activated cation current. The permeability sequence of this cation current for various monovalent cations, as determined from shifts in reversal potential upon extracellular cation substitution, was PK>PCs>PNa>PLi, corresponding to an Eisenman-IV sequence characteristic for a weak-field-strength site. Surprisingly, over-expression of this channel in HEK cells was accompanied by a dramatic down-regulation of the volume-regulated anion channel (VRAC), which is activated by cell swelling in non-transfected cells. In contrast to VRAC, TRP12 could not be activated at constant volume by a reduction of intracellular ionic strength or by intracellular perfusion with guanosine 5'-O-(3-thiotriphosphate (GTPgammaS). The kinetic and pharmacological profile of VRAC and TRP12 currents were also different.
B Nilius; J Prenen; U Wissenbach; M Bödding; G Droogmans
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Pflügers Archiv : European journal of physiology     Volume:  443     ISSN:  0031-6768     ISO Abbreviation:  Pflugers Arch.     Publication Date:  2001 Nov 
Date Detail:
Created Date:  2001-11-19     Completed Date:  2002-04-08     Revised Date:  2006-11-15    
Medline Journal Info:
Nlm Unique ID:  0154720     Medline TA:  Pflugers Arch     Country:  Germany    
Other Details:
Languages:  eng     Pagination:  227-33     Citation Subset:  IM    
Department of Physiology, Campus Gasthuisberg, KU Leuven, Herestraat 49, 3000 Leuven, Belgium.
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MeSH Terms
Anions / metabolism*
Cation Transport Proteins*
Cell Line / cytology
Electric Conductivity
Guanosine 5'-O-(3-Thiotriphosphate) / pharmacology
Ion Channels / physiology*
TRPV Cation Channels
Reg. No./Substance:
0/Anions; 0/Cation Transport Proteins; 0/Ion Channels; 0/Ions; 0/TRPV Cation Channels; 0/TRPV4 protein, human; 37589-80-3/Guanosine 5'-O-(3-Thiotriphosphate)

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