Document Detail

Differential actions of cAMP on endothelial [Ca2+]i and permeability in microvessels exposed to ATP.
MedLine Citation:
PMID:  8214108     Owner:  NLM     Status:  MEDLINE    
We investigated the hypothesis that one of the actions of adenosine 3',5'-cyclic monophosphate (cAMP) to attenuate agonist-induced increase in microvessel permeability is to reduce the initial increase in endothelial cell cytoplasmic Ca2+ concentration ([Ca2+]i). ATP (10 microM) was used to increase hydraulic conductivity (Lp) and [Ca2+]i in venular microvessel in frog mesentery. The cAMP analogue, 8-bromo-cAMP (8-BrcAMP, 2 mM) significantly attenuated the initial increase in Lp (from a peak increase of 7.1 +/- 2.4 times control with ATP alone to 1.2 +/- 0.34 times control with ATP and cAMP). In contrast, cAMP did not significantly change the magnitude or time course of the biphasic increase in [Ca2+]i, which increased from 54 +/- 5 nM to peak of 187 +/- 48 nM with ATP alone, and from 55 +/- 8 nM to 190 +/- 28 nM with ATP and cAMP. Thus the action of cAMP to attenuate microvessel permeability increase in the presence of ATP does not involve modification of the initial Ca2+ transient in endothelial cells. Our results enable alternative hypotheses, such as the suggestion that cAMP might modulate a Ca(2+)-calmodulin-dependent actin-myosin cascade, to be investigated without the added complications of having to account for the modulation of initial changes in [Ca2+]i. Our data also demonstrate, for the first time in the endothelial cells of intact microvessels, that an increase in [Ca2+]i is not always associated with an increase in microvessel permeability.
P He; F E Curry
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't; Research Support, U.S. Gov't, P.H.S.    
Journal Detail:
Title:  The American journal of physiology     Volume:  265     ISSN:  0002-9513     ISO Abbreviation:  Am. J. Physiol.     Publication Date:  1993 Sep 
Date Detail:
Created Date:  1993-11-22     Completed Date:  1993-11-22     Revised Date:  2007-11-14    
Medline Journal Info:
Nlm Unique ID:  0370511     Medline TA:  Am J Physiol     Country:  UNITED STATES    
Other Details:
Languages:  eng     Pagination:  H1019-23     Citation Subset:  IM    
Department of Human Physiology, School of Medicine, University of California, Davis 95616.
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MeSH Terms
Adenosine Triphosphate / pharmacology*
Calcium / metabolism*
Capillary Permeability / drug effects*
Cyclic AMP / pharmacology*
Endothelium, Vascular / metabolism*
Intracellular Membranes / metabolism
Osmolar Concentration
Venules / metabolism
Grant Support
Reg. No./Substance:
56-65-5/Adenosine Triphosphate; 60-92-4/Cyclic AMP; 7440-70-2/Calcium; 96314-98-6/Fura-2

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine

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