Document Detail

Differential accumulation of the mRNA of the auxin-repressed gene CsGRP1 and the auxin-induced peg formation during gravimorphogenesis of cucumber seedlings.
MedLine Citation:
PMID:  16773375     Owner:  NLM     Status:  MEDLINE    
When cucumber seeds are germinated horizontally, an outgrowth (peg) develops on the lower side of the transition zone between the hypocotyl and the root for pulling the cotyledons and plumule out of the seed coat. We previously suggested that gravistimulation suppresses peg formation on the upper side of the transition zone when placed in a horizontal position. In the gravistimulated transition zone, auxin and the mRNAs of auxin-inducible genes are more abundant in the lower side than in the upper side. Here, using fluorescent differential display, we identified Cucumis sativus glycine-rich protein1(CsGRP1) as a gene whose mRNA accumulated more abundantly on the upper side than on the lower side of the transition zone in response to gravistimulation. Auxin starvation increased CsGRP1 mRNA in segments of the transition zone, and inhibition of polar auxin transport with 2,3,5-triiodobenzoic acid (TIBA) prevented the asymmetric accumulation of CsGRP1 mRNA. These results suggest that gravistimulation increases not only the expression of auxin-inducible genes on the lower side of the transition zone, but also the expression of auxin-repressed genes, such as CsGRP1, on the upper side of cucumber seedlings. In the hypocotyls of 3-day-old seedlings, neither gravistimulation nor changes in auxin level influenced the accumulation of CsGRP1 mRNA. These results suggest that the transition zone responds to gravistimulation in a specific manner by an asymmetric expression of CsGRP1 gene during regulation of peg formation.
Minobu Shimizu; Keita Suzuki; Yutaka Miyazawa; Nobuharu Fujii; Hideyuki Takahashi
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't     Date:  2006-06-14
Journal Detail:
Title:  Planta     Volume:  225     ISSN:  0032-0935     ISO Abbreviation:  Planta     Publication Date:  2006 Dec 
Date Detail:
Created Date:  2006-11-19     Completed Date:  2007-03-05     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  1250576     Medline TA:  Planta     Country:  Germany    
Other Details:
Languages:  eng     Pagination:  13-22     Citation Subset:  IM    
Graduate School of Life Sciences, Tohoku University, 2-1-1 Katahira, Aoba-ku, Sendai 980-8577, Japan.
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MeSH Terms
Amino Acid Sequence
Blotting, Northern
Cucumis sativus / drug effects*,  genetics,  metabolism
Gene Expression Regulation, Developmental / drug effects
Gene Expression Regulation, Plant / drug effects
Genes, Plant / genetics
Gravitropism / drug effects*
In Situ Hybridization
Indoleacetic Acids / metabolism,  pharmacology*
Molecular Sequence Data
Plant Growth Regulators / pharmacology
Plant Proteins / genetics*,  metabolism
Plant Roots / drug effects,  genetics,  metabolism
Plant Shoots / drug effects,  genetics,  metabolism
RNA, Messenger / genetics,  metabolism
Reverse Transcriptase Polymerase Chain Reaction
Seedling / drug effects*,  genetics,  growth & development
Sequence Alignment
Reg. No./Substance:
0/Indoleacetic Acids; 0/Plant Growth Regulators; 0/Plant Proteins; 0/RNA, Messenger

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