Document Detail


Differential abilities of capsulated and noncapsulated Staphylococcus aureus isolates from diverse agr groups to invade mammary epithelial cells.
MedLine Citation:
PMID:  17145949     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
Staphylococcus aureus is the bacterium most frequently isolated from milk of bovines with mastitis. Four allelic groups, which interfere with the regulatory activities among the different groups, have been identified in the accessory gene regulator (agr) system. The aim of this study was to ascertain the prevalence of the different agr groups in capsulated and noncapsulated S. aureus bacteria isolated from mastitic bovines in Argentina and whether a given agr group was associated with MAC-T cell invasion and in vivo persistence. Eighty-eight percent of the bovine S. aureus strains were classified in agr group I. The remainder belonged in agr groups II, III, and IV (2, 8, and 2%, respectively). By restriction fragment length polymorphism analysis after PCR amplification of the agr locus variable region, six agr restriction types were identified. All agr group I strains presented a unique allele (A/1), whereas strains from groups II, III, and IV exhibited more diversity. Bovine S. aureus strains defined as being in agr group I (capsulated or noncapsulated) showed significantly increased abilities to be internalized within MAC-T cells, compared with isolates from agr groups II, III, and IV. agr group II or IV S. aureus strains were cleared more efficiently than agr group I strains from the murine mammary gland. The results suggest that agr group I S. aureus strains are more efficiently internalized within epithelial cells and can persist in higher numbers in mammary gland tissue than S. aureus strains classified in agr group II, III, or IV.
Authors:
Fernanda R Buzzola; Lucía P Alvarez; Lorena P N Tuchscherr; María S Barbagelata; Santiago M Lattar; Luis Calvinho; Daniel O Sordelli
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't     Date:  2006-12-04
Journal Detail:
Title:  Infection and immunity     Volume:  75     ISSN:  0019-9567     ISO Abbreviation:  Infect. Immun.     Publication Date:  2007 Feb 
Date Detail:
Created Date:  2007-01-23     Completed Date:  2007-03-08     Revised Date:  2013-06-06    
Medline Journal Info:
Nlm Unique ID:  0246127     Medline TA:  Infect Immun     Country:  United States    
Other Details:
Languages:  eng     Pagination:  886-91     Citation Subset:  IM    
Affiliation:
Departamento de Microbiología, Parasitología e Inmunología, Facultad de Medicina, Universidad de Buenos Aires, Paraguay 2155 p12, C1121ABG Buenos Aires, Argentina.
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MeSH Terms
Descriptor/Qualifier:
Alleles
Animals
Argentina
Bacterial Capsules / physiology*
Bacterial Proteins / genetics*
Cattle
Cell Line
Colony Count, Microbial
DNA Fingerprinting
DNA, Bacterial / genetics
Epithelial Cells / microbiology*
Mammary Glands, Animal / microbiology
Mastitis, Bovine / microbiology*
Mice
Polymorphism, Restriction Fragment Length
Staphylococcal Infections / microbiology,  veterinary*
Staphylococcus aureus / classification,  genetics*,  isolation & purification,  pathogenicity*
Trans-Activators / genetics*
Virulence / genetics
Chemical
Reg. No./Substance:
0/Agr protein, Staphylococcus aureus; 0/Bacterial Proteins; 0/DNA, Bacterial; 0/Trans-Activators
Comments/Corrections

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