| Differential role of Sloan-Kettering Institute (Ski) protein in Nodal and transforming growth factor-beta (TGF-β)-induced Smad signaling in prostate cancer cells. | |
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MedLine Citation:
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PMID: 22843506 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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Transforming growth factor-beta (TGF-β) signaling pathways contain both tumor suppressor and tumor promoting activities. We have demonstrated that Nodal, another member of the TGF-β superfamily, and its receptors are expressed in prostate cancer cells. Nodal and TGF-β exerted similar biological effects on prostate cells; both inhibited proliferation in WPE, RWPE1 and DU145 cells, whereas neither had any effect on the proliferation of LNCaP or PC3 cells. Interestingly, Nodal and TGF-β induced migration in PC3 cells, but not in DU145 cells. TGF-β induced predominantly phosphorylation of Smad3, whereas Nodal induced phosphorylation of only Smad2. We also determined the expression and differential role of Ski, a corepressor of Smad2/3, in Nodal and TGF-β signaling in prostate cancer cells. Similar levels of Ski mRNA were found in several established prostate cell lines; however, high levels of Ski protein were only detected in prostate cancer cells and prostate cancer tissue samples. Exogenous Nodal and TGF-β had no effects on Ski mRNA levels. On the other hand, TGF-β induced a rapid degradation of Ski protein mediated by the proteasomal pathway, whereas Nodal had no effect on Ski protein. Reduced Ski levels correlated with increased basal and TGF-β-induced Smad2/3 phosphorylation. Knockdown of endogenous Ski reduced proliferation in DU145 cells and enhanced migration of PC3 cells. We conclude that high levels of Ski expression in prostate cancer cells may be responsible for repression of TGF-β and Smad3 signaling, but Ski protein levels do not influence Nodal and Smad2 signaling. |
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Authors:
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BaoHan T Vo; Bianca Cody; Yang Cao; Shafiq A Khan |
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Publication Detail:
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Type: Journal Article; Research Support, N.I.H., Extramural; Research Support, U.S. Gov't, Non-P.H.S. Date: 2012-07-27 |
Journal Detail:
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Title: Carcinogenesis Volume: 33 ISSN: 1460-2180 ISO Abbreviation: Carcinogenesis Publication Date: 2012 Nov |
Date Detail:
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Created Date: 2012-10-30 Completed Date: 2013-01-17 Revised Date: 2013-04-16 |
Medline Journal Info:
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Nlm Unique ID: 8008055 Medline TA: Carcinogenesis Country: England |
Other Details:
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Languages: eng Pagination: 2054-64 Citation Subset: IM |
Affiliation:
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Center for Cancer Research and Therapeutic Development, Clark Atlanta University, Atlanta, GA 30314, USA. |
Export Citation:
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APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
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Blotting, Western Cell Movement Cell Proliferation Cells, Cultured DNA-Binding Proteins / antagonists & inhibitors, genetics, metabolism* Fluorescent Antibody Technique Humans Immunoprecipitation Male Nodal Protein / genetics, metabolism* Phosphorylation Prostate / metabolism*, pathology Prostatic Neoplasms / metabolism* Protein Binding Proto-Oncogene Proteins / antagonists & inhibitors, genetics, metabolism* RNA, Messenger / genetics RNA, Small Interfering / genetics Real-Time Polymerase Chain Reaction Reverse Transcriptase Polymerase Chain Reaction Signal Transduction Smad2 Protein / genetics, metabolism* Smad3 Protein / genetics, metabolism* Transforming Growth Factor beta / genetics, metabolism* Wound Healing |
| Grant Support | |
ID/Acronym/Agency:
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2G12RR003062/RR/NCRR NIH HHS; 2R25GM060414/GM/NIGMS NIH HHS; 5P20MD002285-02/MD/NIMHD NIH HHS |
| Chemical | |
Reg. No./Substance:
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0/DNA-Binding Proteins; 0/NODAL protein, human; 0/Nodal Protein; 0/Proto-Oncogene Proteins; 0/RNA, Messenger; 0/RNA, Small Interfering; 0/SMAD2 protein, human; 0/SMAD3 protein, human; 0/Smad2 Protein; 0/Smad3 Protein; 0/Transforming Growth Factor beta; 126648-96-2/SKI protein, human |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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