| Differential Regulation of ROMK (Kir1.1) in Distal Nephron Segments by Dietary Potassium. | |
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MedLine Citation:
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PMID: 21454252 Owner: NLM Status: Publisher |
Abstract/OtherAbstract:
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ROMK channels are well known to play a central role in renal K secretion, but the absence of highly specific and avid-ROMK antibodies has presented significant roadblocks toward mapping the extent of expression along the entire distal nephron and determining whether surface density of these channels is regulated in response to physiological stimuli. Here, we prepared new ROMK antibodies verified to be highly specific, using ROMK knockout mice as a control. Characterization with segmental markers revealed a more extensive pattern of ROMK expression along the entire distal nephron than previously thought, localizing to distal convoluted tubule regions, DCT1 and DCT2; the connecting tubule (CNT); and cortical collecting duct (CD). ROMK was diffusely distributed in intracellular compartments and at the apical membrane of each tubular region. Apical labeling was significantly increased by high K diet in DCT2, CNT1, CNT2 and CD (P<0.05) but not in DCT1. Consistent with the large increase in apical ROMK, dramatically increased mature glycosylation was observed following dietary potassium augmentation. We conclude: 1) our new antibody provides a unique tool to characterize ROMK channel localization and expression; 2) High K diet causes a large increase in apical expression of ROMK in DCT2, CNT and CD but not in DCT1, indicating that different regulatory mechanisms are involved in K diet regulated ROMK channel functions in the distal nephron. |
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Authors:
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James B Wade; Liang Fang; Richard A Coleman; Jie Liu; P Richard Grimm; Tong Wang; Paul A Welling |
Publication Detail:
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Type: JOURNAL ARTICLE Date: 2011-3-30 |
Journal Detail:
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Title: American journal of physiology. Renal physiology Volume: - ISSN: 1522-1466 ISO Abbreviation: - Publication Date: 2011 Mar |
Date Detail:
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Created Date: 2011-4-1 Completed Date: - Revised Date: - |
Medline Journal Info:
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Nlm Unique ID: 100901990 Medline TA: Am J Physiol Renal Physiol Country: - |
Other Details:
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Languages: ENG Pagination: - Citation Subset: - |
Affiliation:
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1University of Maryland. |
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