| Differential regulation of caspase-1 activation via NLRP3/NLRC4 inflammasomes mediated by aerolysin and type III secretion system during Aeromonas veronii infection. | |
| | |
MedLine Citation:
|
PMID: 21037094 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
|
Aeromonas spp. are Gram-negative bacteria that cause serious infectious disease in humans. Such bacteria have been shown to induce apoptosis in infected macrophages, yet the host responses triggered by macrophage death are largely unknown. In this study, we demonstrate that the infection of mouse bone marrow-derived macrophages with Aeromonas veronii biotype sobria triggers activation of caspase-1 with the ensuing release of IL-1β and pyroptosis. Caspase-1 activation in response to A. veronii infection requires the adaptor apoptosis-associated speck-like protein containing a caspase recruitment domain and both the NLRP3 and NLRC4 inflammasomes. Furthermore, caspase-1 activation requires aerolysin and a functional type III secretion system in A. veronii. Aerolysin-inducing caspase-1 activation is mediated through the NLRP3 inflammasome, with aerolysin-mediated cell death being largely dependent on the NLRP3 inflammasome. In contrast, the type III secretion system activates both the NLRP3 and NLRC4 inflammasomes. Inflammasome-mediated caspase-1 activation is also involved in host defenses against systemic A. veronii infection in mice. Our results indicated that multiple factors from both the bacteria and the host play a role in eliciting caspase-1 activation during A. veronii infection. |
| | |
Authors:
|
Andrea J McCoy; Yukiko Koizumi; Naomi Higa; Toshihiko Suzuki |
Related Documents
:
|
15737884 - Modification of iron regulation by the inflammatory response. 20961764 - What is type vi secretion doing in all those bugs? 15329444 - Regulation by innate immune t lymphocytes in the host defense against pulmonary infecti... 15972584 - Lipopolysaccharide heterogeneity: innate host responses to bacterial modification of li... 8384974 - Impact of hematopoietic growth factors on the management of small-cell lung cancer. 22288454 - Th17 cells: interactions with predisposing factors in the immunopathogenesis of inflamm... |
Publication Detail:
|
Type: Comparative Study; Journal Article; Research Support, Non-U.S. Gov't Date: 2010-10-29 |
Journal Detail:
|
Title: Journal of immunology (Baltimore, Md. : 1950) Volume: 185 ISSN: 1550-6606 ISO Abbreviation: J. Immunol. Publication Date: 2010 Dec |
Date Detail:
|
Created Date: 2010-11-18 Completed Date: 2011-01-10 Revised Date: - |
Medline Journal Info:
|
Nlm Unique ID: 2985117R Medline TA: J Immunol Country: United States |
Other Details:
|
Languages: eng Pagination: 7077-84 Citation Subset: AIM; IM |
Affiliation:
|
Department of Molecular Bacteriology and Immunology, Graduate School of Medicine, University of the Ryukyus, Nishiharacho, Okinawa, Japan. |
Export Citation:
|
APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
|
Aeromonas
/
immunology*,
pathogenicity Animals Apoptosis Regulatory Proteins / deficiency, genetics, physiology* Bacterial Secretion Systems / immunology* Bacterial Toxins / biosynthesis Bone Marrow Cells / immunology, microbiology, pathology Calcium-Binding Proteins / deficiency, genetics, physiology* Carrier Proteins / genetics, physiology* Caspase 1 / deficiency, genetics, metabolism* Cell Death / genetics, immunology Cells, Cultured Gram-Negative Bacterial Infections / enzymology, genetics, immunology Inflammasomes / deficiency, genetics, physiology* Macrophages / immunology, microbiology, pathology Mice Mice, Inbred C57BL Mice, Knockout Pore Forming Cytotoxic Proteins / biosynthesis, physiology* |
| Chemical | |
Reg. No./Substance:
|
0/Apoptosis Regulatory Proteins; 0/Bacterial Toxins; 0/CIAS1 protein, mouse; 0/Calcium-Binding Proteins; 0/Carrier Proteins; 0/Inflammasomes; 0/Ipaf protein, mouse; 0/Pore Forming Cytotoxic Proteins; 53126-24-2/aerolysin; EC 3.4.22.36/Caspase 1 |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
Previous Document: Chlamydia-specific CD4 T cell clones control Chlamydia muridarum replication in epithelial cells by ...
Next Document: IL-2 complex treatment can protect naive mice from bacterial and viral infection.