| Differential intra-abdominal adipose tissue profiling in obese, insulin-resistant women. | |
| | |
MedLine Citation:
|
PMID: 19711137 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
|
BACKGROUND: We recently identified differences in abdominal subcutaneous adipose tissue (SAT) from insulin-resistant (IR) as compared to obesity-matched insulin sensitive individuals, including accumulation of small adipose cells, decreased expression of cell differentiation markers, and increased inflammatory activity. This study was initiated to see if these changes in SAT of IR individuals were present in omental visceral adipose tissue (VAT); in this instance, individuals were chosen to be IR but varied in degree of adiposity. We compared cell size distribution and genetic markers in SAT and VAT of IR individuals undergoing bariatric surgery. METHODS: Eleven obese/morbidly obese women were IR by the insulin suppression test. Adipose tissue surgical samples were fixed in osmium tetroxide for cell size analysis via Beckman Coulter Multisizer. Quantitative real-time polymerase chain reaction for genes related to adipocyte differentiation and inflammation was performed. RESULTS: While proportion of small cells and expression of adipocyte differentiation genes did not differ between depots, inflammatory genes were upregulated in VAT. Diameter of SAT large cells correlated highly with increasing proportion of small cells in both SAT and VAT (r = 0.85, p = 0.001; r = 0.72, p = 0.01, respectively). No associations were observed between VAT large cells and cell size variables in either depot. The effect of body mass index (BMI) on any variables in both depots was negligible. CONCLUSIONS: The major differential property of VAT of IR women is increased inflammatory activity, independent of BMI. The association of SAT adipocyte hypertrophy with hyperplasia in both depots suggests a primary role SAT may have in regulating regional fat storage. |
| | |
Authors:
|
Alice Liu; Tracey McLaughlin; Teresa Liu; Arthur Sherman; Gail Yee; Fahim Abbasi; Cindy Lamendola; John Morton; Samuel W Cushman; Gerald M Reaven; Philip S Tsao |
Related Documents
:
|
11262197 - Transcriptional activation of prion protein gene in growth-arrested and differentiated ... 2643857 - Studies of the proliferation and differentiation of immature myeloid cells in vitro. i.... 12869207 - Maintainence of parasitaemia - is it to die for? 14643407 - Differentiation of fonsecaea pedrosoi mycelial forms into sclerotic cells is induced by... 21711857 - Biocompatibility of hydrophilic silica-coated cdte quantum dots and magnetic nanopartic... 11262197 - Transcriptional activation of prion protein gene in growth-arrested and differentiated ... |
Publication Detail:
|
Type: Comparative Study; Journal Article; Research Support, N.I.H., Extramural; Research Support, N.I.H., Intramural Date: 2009-08-27 |
Journal Detail:
|
Title: Obesity surgery Volume: 19 ISSN: 1708-0428 ISO Abbreviation: Obes Surg Publication Date: 2009 Nov |
Date Detail:
|
Created Date: 2009-10-28 Completed Date: 2010-03-19 Revised Date: 2012-05-07 |
Medline Journal Info:
|
Nlm Unique ID: 9106714 Medline TA: Obes Surg Country: United States |
Other Details:
|
Languages: eng Pagination: 1564-73 Citation Subset: IM |
Affiliation:
|
Division of Endocrinology, Department of Medicine, Stanford University Medical Center, 300 Pasteur Drive Rm S-025, Stanford, CA 94305, USA. aliceliu@stanford.edu |
Export Citation:
|
APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
|
Adipocytes
/
cytology,
physiology Adipogenesis / physiology* Adult Body Mass Index Cell Size Female Gene Expression Humans Immunohistochemistry Inflammation / metabolism Insulin Resistance* Intra-Abdominal Fat / cytology*, metabolism* Middle Aged Obesity / genetics, metabolism* Obesity, Morbid / genetics, metabolism Subcutaneous Fat, Abdominal / cytology*, metabolism* Young Adult |
| Grant Support | |
ID/Acronym/Agency:
|
1 R01 DK071309-01/DK/NIDDK NIH HHS; 5 F32 DK079578-02/DK/NIDDK NIH HHS; 5 R01 DK071333-04/DK/NIDDK NIH HHS; F32 DK079578-01/DK/NIDDK NIH HHS; F32 DK079578-02/DK/NIDDK NIH HHS; M01-RR00070/RR/NCRR NIH HHS; R01 DK080436-03/DK/NIDDK NIH HHS |
| Comments/Corrections | |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
Previous Document: Use of colon cancer testing in rural colorado primary care practices.
Next Document: Safety and Short-Term Outcomes of Laparoscopic Sleeve Gastrectomy as a Revisional Approach for Faile...