Document Detail

Differential effects of G- and F-actin on the plasma membrane calcium pump activity.
MedLine Citation:
PMID:  23152090     Owner:  NLM     Status:  MEDLINE    
We have previously shown that plasma membrane calcium ATPase (PMCA) pump activity is affected by the membrane protein concentration (Vanagas et al., Biochim Biophys Acta 1768:1641-1644, 2007). The results of this study provided evidence for the involvement of the actin cytoskeleton. In this study, we explored the relationship between the polymerization state of actin and its effects on purified PMCA activity. Our results show that PMCA associates with the actin cytoskeleton and this interaction causes a modulation of the catalytic activity involving the phosphorylated intermediate of the pump. The state of actin polymerization determines whether it acts as an activator or an inhibitor of the pump: G-actin and/or short oligomers activate the pump, while F-actin inhibits it. The effects of actin on PMCA are the consequence of direct interaction as demonstrated by immunoblotting and cosedimentation experiments. Taken together, these findings suggest that interactions with actin play a dynamic role in the regulation of PMCA-mediated Ca(2+) extrusion through the membrane. Our results provide further evidence of the activation-inhibition phenomenon as a property of many cytoskeleton-associated membrane proteins where the cytoskeleton is no longer restricted to a mechanical function but is dynamically involved in modulating the activity of integral proteins with which it interacts.
Laura Vanagas; María Candelaria de La Fuente; Marianela Dalghi; Mariela Ferreira-Gomes; Rolando C Rossi; Emanuel E Strehler; Irene C Mangialavori; Juan P F C Rossi
Publication Detail:
Type:  Journal Article; Research Support, N.I.H., Extramural; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Cell biochemistry and biophysics     Volume:  66     ISSN:  1559-0283     ISO Abbreviation:  Cell Biochem. Biophys.     Publication Date:  2013 May 
Date Detail:
Created Date:  2013-04-16     Completed Date:  2013-12-19     Revised Date:  2014-05-08    
Medline Journal Info:
Nlm Unique ID:  9701934     Medline TA:  Cell Biochem Biophys     Country:  United States    
Other Details:
Languages:  eng     Pagination:  187-98     Citation Subset:  IM    
Export Citation:
APA/MLA Format     Download EndNote     Download BibTex
MeSH Terms
Actin Cytoskeleton
Actins / chemistry*,  classification
Calcium / chemistry*
Calcium Signaling*
Calcium-Transporting ATPases / chemistry*
Enzyme Activation
Erythrocyte Membrane / chemistry,  enzymology*
Erythrocytes / chemistry,  enzymology
Membrane Proteins / chemistry
Protein Conformation
Grant Support
Reg. No./Substance:
0/Actins; 0/Membrane Proteins; EC ATPases; SY7Q814VUP/Calcium

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine

Previous Document:  Prognostic factors of sciatica in the Canon of Avicenna.
Next Document:  Modulation of Chitotriosidase During Macrophage Differentiation.