Document Detail

Differential DNA strand breaking abilities of *OH and ROS generating radiomimetic chemicals and gamma-rays: study of plasmid DNA, pMTa4, in vitro.
MedLine Citation:
PMID:  16036325     Owner:  NLM     Status:  MEDLINE    
Reactive oxygen species (ROS) interact with components of a living cell. Among them *OH is known to cause major oxidative damage to living cells and is proposed to be involved in pathogenesis including carcinogenesis. Proper understanding of consequences of such damage is, therefore, medically relevant. In this report, aqueous solution of plasmid DNA, pMTa4, has been exposed to Fenton oxidant and Haber-Weiss oxidant mediated free radical generating chemical systems, and 60Co gamma-rays in vitro either alone or in combination to study their strand breaking abilities. The exposed pMTa4 was analyzed by agarose gel electrophoresis. The results show qualitative differences in the induction of strand breaks on the plasmid pMTa4 molecule by the iron (Fe2+), copper (Cu2+) or gamma-rays mediated *OH and other ROS.
M Meriyani Odyuo; R N Sharan
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Publication Detail:
Type:  Comparative Study; Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Free radical research     Volume:  39     ISSN:  1071-5762     ISO Abbreviation:  Free Radic. Res.     Publication Date:  2005 May 
Date Detail:
Created Date:  2005-07-22     Completed Date:  2005-09-29     Revised Date:  2006-11-15    
Medline Journal Info:
Nlm Unique ID:  9423872     Medline TA:  Free Radic Res     Country:  England    
Other Details:
Languages:  eng     Pagination:  499-505     Citation Subset:  IM    
Department of Biochemistry, North-Eastern Hill University, Radiation & Molecular Biology Unit, Shillong, 793 022, India.
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MeSH Terms
Chelating Agents / pharmacology
Copper / adverse effects
DNA Damage / drug effects*,  radiation effects*
Electrophoresis, Agar Gel
Free Radical Scavengers / pharmacology
Gamma Rays / adverse effects*
Hydrogen Peroxide / adverse effects
Hydroxyl Radical / adverse effects*
Iron / adverse effects
Plasmids / drug effects,  radiation effects
Reactive Oxygen Species / adverse effects*
Reg. No./Substance:
0/Chelating Agents; 0/Fenton's reagent; 0/Free Radical Scavengers; 0/Reactive Oxygen Species; 3352-57-6/Hydroxyl Radical; 7439-89-6/Iron; 7440-50-8/Copper; 7722-84-1/Hydrogen Peroxide

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