Document Detail


Differential Clustering of Sperm Subpopulations in Infertile Males with Clinical Varicocele and Carriers of Rearranged Genomes.
MedLine Citation:
PMID:  21835912     Owner:  NLM     Status:  Publisher    
Abstract/OtherAbstract:
Some methods for determining sperm DNA fragmentation, such as the Sperm Chromatin Structure Assay (SCSA) and the Sperm Chromatin Dispersion test (SCD), provide additional information about particular subgroups of spermatozoa with specific irregularities. Thus, SCSA recognizes a specific sperm subpopulation, the High DNA Stainability sperm subpopulation (HDS), and SCD recognizes the so-called DNA Degraded Sperm (DDS) subpopulation. Although some studies associate the presence of these subpopulations with specific aspects related to infertility, the relationship between both sperm subpopulations and their preponderance in specific clinical groups of infertile males has not been extensively investigated. In this study, HDS and DDS subpopulations were determined in a total of 37 human males: eight males with proven fertility, nine infertile males with asthenoteratozoospermia (ATZ), ten carriers of chromosomal reorganizations and ten infertile males with clinical varicocele. Results showed a significant increase of the DDS subpopulation (p<0.001) in both varicocele patient (16.85±7.24) and carriers of rearranged genome (11.6±5.23) groups, but not in ATZ patients (3.88±1.55) or fertile donors (2.62±1.68). No statistical differences were detected for the HDS subpopulation (p=0.542), but the highest values were found in varicocele and rearranged genome groups. However, no correlation between the HDS and DDS subpopulations were found (r=0.196; p=0.244), suggesting that both represent a different class of sperm subpopulation in the ejaculate. A significant increase in HDS, and especially DDS, can be associated with the presence of varicocele or the rearrangement of chromosomes. Specific diagnostic tests to confirm the diagnosis must be performed in patients with increased DDS and HDS values.
Authors:
Agustín García-Peiró; María Oliver-Bonet; Joaquima Navarro; Carlos Abad; María José Amengual; Carmen López-Fernández; Jaime Gosálvez; Jordi Benet
Publication Detail:
Type:  JOURNAL ARTICLE     Date:  2011-8-11
Journal Detail:
Title:  Journal of andrology     Volume:  -     ISSN:  1939-4640     ISO Abbreviation:  -     Publication Date:  2011 Aug 
Date Detail:
Created Date:  2011-8-12     Completed Date:  -     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  8106453     Medline TA:  J Androl     Country:  -    
Other Details:
Languages:  ENG     Pagination:  -     Citation Subset:  -    
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