Document Detail


Differential Apoptosis in Mucosal and Dermal Wound Healing.
MedLine Citation:
PMID:  25493209     Owner:  NLM     Status:  Publisher    
Abstract/OtherAbstract:
Objectives: Dermal and mucosal healing are mechanistically similar. However, scarring and closure rates are dramatically improved in mucosal healing, possibly due to differences in apoptosis. Apoptosis, nature's preprogrammed form of cell death, occurs via two major pathways, extrinsic and intrinsic, which intersect at caspase3 (Casp3) cleavage and activation. The purpose of this experiment was to identify the predominant pathways of apoptosis in mucosal and dermal wound healing. Approach: Wounds (1 mm biopsy punch) were made in the dorsal skin (n=3) or tongue (n=3) of female Balb/C mice aged 6 weeks. Wounds were harvested at 6 h, 24 h, day 3 (D3), D5, D7, and D10. RNA was isolated and analyzed using real time reverse transcriptase-polymerase chain reaction. Expression levels for genes in the intrinsic and extrinsic apoptotic pathways were compared in dermal and mucosal wounds. Results: Compared to mucosal healing, dermal wounds exhibited significantly higher expression of Casp3 (at D5; p<0.05), Casp7 (at D5; p<0.05), Trp53 (at 24 h and D5; p<0.05), Tnfrsf1b (at 24 h; p<0.05), FasR (at 24 h, D5, and D7; p<0.05), and Casp8 (at 24 h; p<0.05) and significantly lower gene expression of Tradd (at 24 h; p<0.05). Innovation: Our observations indicate differential execution of apoptosis in oral wound healing compared to skin. Conclusion: Expression patterns of key regulators of apoptosis in wound healing indicate that apoptosis occurs predominantly through the intrinsic pathway in the healing mucosa, but predominantly through the extrinsic pathway in the healing skin. The identification of differences in the apoptotic pathways in skin and mucosal wounds may allow the development of therapeutics to improve skin healing.
Authors:
Ariel Johnson; Marybeth Francis; Luisa Ann DiPietro
Related Documents :
9580669 - Csr, a scavenger receptor-like protein with a protective role against cellular damage c...
15709959 - Endocrinology of the stress response.
10823909 - Shear stress-induced apoptosis of adherent neutrophils: a mechanism for persistence of ...
25381719 - Ginsenoside f1 attenuates hyperpigmentation in b16f10 melanoma cells by inducing dendri...
17582219 - The roles of synoviolin in crosstalk between endoplasmic reticulum stress-induced apopt...
19883139 - Stress and specificity: reply to miller (2009).
15605989 - Ischemia leads to apoptosis--and necrosis-like neuron death in the ischemic rat hippoca...
23954699 - Osteoimmunology in mucopolysaccharidoses type i, ii, vi and vii. immunological regulati...
14691289 - Pioglitazone, a peroxisome proliferator-activated receptor-gamma agonist, attenuates my...
Publication Detail:
Type:  JOURNAL ARTICLE    
Journal Detail:
Title:  Advances in wound care     Volume:  3     ISSN:  2162-1918     ISO Abbreviation:  Adv Wound Care (New Rochelle)     Publication Date:  2014 Dec 
Date Detail:
Created Date:  2014-12-10     Completed Date:  -     Revised Date:  2014-12-11    
Medline Journal Info:
Nlm Unique ID:  101590593     Medline TA:  Adv Wound Care (New Rochelle)     Country:  -    
Other Details:
Languages:  ENG     Pagination:  751-761     Citation Subset:  -    
Export Citation:
APA/MLA Format     Download EndNote     Download BibTex
MeSH Terms
Descriptor/Qualifier:

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


Previous Document:  Strategies for Oral Mucosal Repair by Engineering 3D Tissues with Pluripotent Stem Cells.
Next Document:  Integrins in Wound Healing.