Document Detail


Different types of mitochondria in parenchymal and non-parenchymal rat-liver cells.
MedLine Citation:
PMID:  190009     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
1. Intact and pure parenchymal and non-parenchymal cells were isolated from rat liver. The specific activities of several mitochondrial enzymes were determined in both parenchymal and non-parenchymal cell homogenates to characterize the mitochondria in these liver cell types. 2. In general the activities of mitochondrial enzymes were lower in non-parenchymal liver cells than in parenchymal cells. The specific activity of pyruvate carboxylase in non-parenchymal cells expressed as the percentage of that in parenchymal cells was onlu 2% for glutamate dehydrogenase 4.3% and for cytochrome c oxidase 79.4%. Monoamine oxidase, as an exception, has an equal specific activity in both cell types. 3. The activity ratio of pyruvate carboxylase at 10 mM pyruvate over 0.1 mM pyruvate is 3.35 for parenchymal cells and 1.50 for non-parenchymal cells. This indicates that non-parenchymal liver cells only contain the high affinity form of pyruvate carboxylase in contrast to parenchymal cells. 4. The ratio of glycerol-3-phosphate cytochrome c reductase over succinate cytochrome c reductase activity differs from parenchymal (0.01) and non-parenchymal cells (0.10). This might indicate that the glycerol-3-phosphate shuttle, which is important for the transport of reduction equivalents for cytosol to mitochondria is relatively more active in non-parenchymal cells than in parenchymal cells. 5. The activity pattern of mitochondrial enzymes in parenchymal and non-parenchymal cell homogenates indicates that these cell types contain different types of mitochondria. The presence of these different cell types in liver will therefore contribute to the heterogeneity of isolated rat liver mitochondria in which the mitochondria from non-parenchymal cells might be considered as "non-gluconeogenic".
Authors:
T J Van Berkel; J K Kruijt
Related Documents :
6371179 - The origin of intrahepatic bile duct cells in the mouse.
7085759 - Protein synthesis in different populations of rat hepatocytes separated according to de...
9537259 - Role of nitric oxide and superoxide anion in elimination of low metastatic human colore...
11310849 - Adenoviral gene delivery can inactivate kupffer cells: role of oxidants in nf-kappab ac...
10905589 - The immunohistochemical phenotype of dysplastic foci in human liver: correlation with p...
3884999 - Insulin uptake by rat liver endothelium studied in fractionated liver cell suspensions.
19872319 - Some physicochemical properties of dissociated sponge cells.
22738959 - Enhanced immunomodulatory activity and stability in simulated digestive juices of lacto...
10577389 - Induction of s phase by g1 regulatory factors.
Publication Detail:
Type:  Journal Article    
Journal Detail:
Title:  European journal of biochemistry / FEBS     Volume:  73     ISSN:  0014-2956     ISO Abbreviation:  Eur. J. Biochem.     Publication Date:  1977 Feb 
Date Detail:
Created Date:  1977-04-30     Completed Date:  1977-04-30     Revised Date:  2009-11-03    
Medline Journal Info:
Nlm Unique ID:  0107600     Medline TA:  Eur J Biochem     Country:  GERMANY, WEST    
Other Details:
Languages:  eng     Pagination:  223-9     Citation Subset:  IM    
Export Citation:
APA/MLA Format     Download EndNote     Download BibTex
MeSH Terms
Descriptor/Qualifier:
Animals
Cell Fractionation
Cytochrome Reductases / metabolism
Electron Transport Complex IV / metabolism
Glutamate Dehydrogenase / metabolism
Glycerolphosphate Dehydrogenase / metabolism
Liver / enzymology
Mitochondria, Liver / enzymology*,  ultrastructure
Pyruvate Carboxylase / metabolism
Pyruvate Kinase / metabolism
Rats
Succinate Dehydrogenase / metabolism
Chemical
Reg. No./Substance:
EC 1.1.-/Glycerolphosphate Dehydrogenase; EC 1.3.99.1/Succinate Dehydrogenase; EC 1.4.1.2/Glutamate Dehydrogenase; EC 1.6.2.-/Cytochrome Reductases; EC 1.9.3.1/Electron Transport Complex IV; EC 2.7.1.40/Pyruvate Kinase; EC 6.4.1.1/Pyruvate Carboxylase

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


Previous Document:  Evidence for the identity of nuclear and cytoplasmic adenosine-3':5'-monophosphate-dependent protein...
Next Document:  Effect of hyperoxide radicals on bovine-erythrocyte membrane.