Document Detail


Different sensitivity of neurons and neuroblastoma cells to quercetin treatment.
MedLine Citation:
PMID:  19112469     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
Quercetin is a natural flavonoid with pro-apoptotic and antiproliferative properties. In this study, we determined the sensitivity of neurons and neuroblastoma cells on apoptosis and necrosis induction upon quercetin treatment. No expression of Hsp72 was observed in neurons, which were more sensitive to cell death upon quercetin treatment than neuroblastoma cells, where Hsp72 expression was observed. Reduction of Hsp72 gene expression in neuroblastoma cells by antisense oligonucleotides made them more sensitive to pro-apoptotic action of quercetin. Moreover, the flavonoid decreased Hsp27, procaspase-3, MRP and PKB expression in neuroblastoma cells and in neurons. Nuclear localization of mainly cytoplasmic Hsp27 was observed in neuroblastoma cells after treatment with high quercetin concentrations, while in neurons, the protein was present in nuclei both in control and quercetin treated cells. Our results suggest that quercetin induce apoptosis more effectively in cells with low level of Hsp 72 expression. Higher sensitivity of neurons for cell death after treatment with high quercetin concentrations in comparison to neuroblastoma cell line should also be taken into consideration in further studies on using studied flavonoid as therapeutic agent.
Authors:
Joanna Jakubowicz-Gil; Wojciech Rzeski; Barbara Zdzisińska; Tomasz Piersiak; Katarzyna Weiksza; Kazimierz Glowniak; Antoni Gawron
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Publication Detail:
Type:  Journal Article    
Journal Detail:
Title:  Acta neurobiologiae experimentalis     Volume:  68     ISSN:  0065-1400     ISO Abbreviation:  Acta Neurobiol Exp (Wars)     Publication Date:  2008  
Date Detail:
Created Date:  2008-12-29     Completed Date:  2009-03-13     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  1246675     Medline TA:  Acta Neurobiol Exp (Wars)     Country:  Poland    
Other Details:
Languages:  eng     Pagination:  463-76     Citation Subset:  IM    
Affiliation:
Department of Comparative Anatomy and Anthropology, Maria Curie-Skłodowska University, Lublin, Poland. asiajgil@02.pl
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MeSH Terms
Descriptor/Qualifier:
Animals
Apoptosis / drug effects
Blotting, Western
Brain Neoplasms / pathology*
Cell Line, Tumor
Cells, Cultured
HSP72 Heat-Shock Proteins / metabolism
Humans
Necrosis
Nerve Tissue Proteins / metabolism
Neuroblastoma / pathology*
Neurons / drug effects*
Quercetin / pharmacology*
Rats
Rats, Wistar
Transfection
Chemical
Reg. No./Substance:
0/HSP72 Heat-Shock Proteins; 0/Nerve Tissue Proteins; 117-39-5/Quercetin

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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