| Different radiosensitivity of CD4(+)CD25(+) regulatory T cells and effector T cells to low dose gamma irradiation in vitro. | |
| | |
MedLine Citation:
|
PMID: 20958220 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
|
PURPOSE: To determine the radiosensitivity difference of human Cluster of Differentiation (CD)4(+)CD25(+) regulatory T cells (Treg) and effector T cells to low dose gamma ray and elucidate the underlying mechanisms in vitro. MATERIALS AND METHODS: Blood samples were collected from five health subjects and five patients with advanced hepatocellular carcinoma (HCC). Treg and CD4(+)CD25⁻ T cells were selected using magnetic microbeads. The proliferative profiles, cytokine secretion, and differential expressions of apoptosis-related proteins in Treg and CD4(+)CD25⁻ T cells were compared using [³H]-thymidine incorporation, Luminex assay and flow cytometry when treated with various low doses of γ-ray. RESULTS: A dose-dependent reduction of proliferation in response to irradiation which paralleled the induction of apoptosis existed in Treg and CD4(+)CD25⁻ T cells. Treg were more radiosensitive to low-dose irradiation (0.94 Gray [Gy]) than effector T cells. The interferon-γ (IFNγ) was significantly upregulated and interleukin 10 (IL-10) was significantly downregulated in irradiated Treg. An enhanced immune response to low dose gamma ray existed in the peripheral blood in patients with advanced HCC. Higher levels of active caspase-3, CD95, B cell lymphoma 2 (Bcl-2)-associated X protein (Bax) expression were observed in Treg compared to CD4(+)CD25⁻ T cells. In addition, gamma irradiation activated CD4(+)CD25⁻ T cells to express CD25. CONCLUSIONS: These studies revealed that Treg were more radiosensitive than CD4(+)CD25⁻ T cells to low dose irradiation. Higher expressions of apoptosis-related proteins such as caspase-3, CD95 and Bax were observed in Treg when compared to CD4(+)CD25⁻ T cells. Our results suggest that treatment with low doses of gamma irradiation may be a viable strategy to enhance immune response in patients with advanced HCC. |
| | |
Authors:
|
Mengde Cao; Roniel Cabrera; Yiling Xu; Chen Liu; David Nelson |
Publication Detail:
|
Type: In Vitro; Journal Article; Research Support, N.I.H., Extramural Date: 2010-10-20 |
Journal Detail:
|
Title: International journal of radiation biology Volume: 87 ISSN: 1362-3095 ISO Abbreviation: Int. J. Radiat. Biol. Publication Date: 2011 Jan |
Date Detail:
|
Created Date: 2010-12-14 Completed Date: 2011-01-24 Revised Date: - |
Medline Journal Info:
|
Nlm Unique ID: 8809243 Medline TA: Int J Radiat Biol Country: England |
Other Details:
|
Languages: eng Pagination: 71-80 Citation Subset: IM; S |
Affiliation:
|
Section of Hepatobiliary Diseases, Department of Medicine, University of Florida, Gainesville, FL 32610-0214, USA. caom@medicine.ufl.edu |
Export Citation:
|
APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
|
Aged Antigens, CD95 / metabolism Apoptosis / radiation effects Carcinoma, Hepatocellular / immunology, pathology, radiotherapy Caspase 3 / metabolism Cell Proliferation / radiation effects Dose-Response Relationship, Radiation Female Gamma Rays / therapeutic use* Humans Interferon-gamma / biosynthesis Interleukin-10 / biosynthesis Liver Neoplasms / immunology, pathology, radiotherapy Male Middle Aged Radiation Tolerance / immunology T-Lymphocyte Subsets / immunology*, metabolism, pathology, radiation effects* T-Lymphocytes, Regulatory / immunology*, metabolism, pathology, radiation effects* bcl-2-Associated X Protein / metabolism |
| Grant Support | |
ID/Acronym/Agency:
|
AI061158/AI/NIAID NIH HHS; DK 60443/DK/NIDDK NIH HHS; RR00082/RR/NCRR NIH HHS |
| Chemical | |
Reg. No./Substance:
|
0/Antigens, CD95; 0/BAX protein, human; 0/FAS protein, human; 0/IL10 protein, human; 0/bcl-2-Associated X Protein; 130068-27-8/Interleukin-10; 82115-62-6/Interferon-gamma; EC 3.4.22.-/CASP3 protein, human; EC 3.4.22.-/Caspase 3 |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
Previous Document: Short and long term anti-inflammatory effects of bosentan therapy in patients with pulmonary arteria...
Next Document: Mito-communications.