Document Detail

Different profile of interleukin-10 production in circulating T cells from atopic asthmatics compared with healthy subjects.
MedLine Citation:
PMID:  15010730     Owner:  NLM     Status:  MEDLINE    
BACKGROUND: Interleukin (IL)-10 is a pleiotropic cytokine released from various cells, including T cells. Although IL-10 is suggested to inhibit allergic responses, its role in asthma remains uncertain. The purpose of the present study was to compare the profile of IL-10 in circulating T cells from stable atopic asthmatics, atopic nonasthmatics and healthy controls. METHODS: Peripheral blood mononuclear cells were isolated, stained with anti-CD3 and CD4/CD8 antibodies, and then processed for intracellular IL-10 detection by flow cytometry. RESULTS: A kinetic study in healthy controls showed that stimulation with phorbol 12-myristate 13-acetate and ionomycin significantly increased the frequencies of IL-10-producing CD3+, CD4+ and CD8+ cells. Without stimulation, the frequencies of IL-10-producing CD3+, CD4+ and CD8+cells were significantly higher in asthmatics than in healthy controls, while a similar trend was observed in atopic nonasthmatics. Stimulation for 24 h significantly increased IL-10-producing CD3+, CD4+ and CD8+cells in healthy controls and atopic nonasthmatics, but not in asthmatics. CONCLUSIONS: The frequency of IL-10-producing T cells is increased in the circulation of stable atopic asthmatics compared with normal controls. The lack of enhancement in their frequency by phorbol 12-myristate 13-acetate and ionomycin in asthmatics suggests that the circulating T cells of asthmatic subjects are maximally stimulated with regards to IL-10 production; alternatively, IL-10 production by T cells from asthmatics may be regulated differently than T cells from other subjects.
K Matsumoto; S Narita; T Rerecich; D P Snider; P M O'Byrne
Publication Detail:
Type:  Clinical Trial; Comparative Study; Controlled Clinical Trial; Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Canadian respiratory journal : journal of the Canadian Thoracic Society     Volume:  11     ISSN:  1198-2241     ISO Abbreviation:  Can. Respir. J.     Publication Date:    2004 Jan-Feb
Date Detail:
Created Date:  2004-03-10     Completed Date:  2004-08-09     Revised Date:  2006-11-15    
Medline Journal Info:
Nlm Unique ID:  9433332     Medline TA:  Can Respir J     Country:  Canada    
Other Details:
Languages:  eng     Pagination:  33-8     Citation Subset:  IM    
Asthma Research Group, Firestone Institute of Respiratory Health, St Joseph's Healthcare, McMaster University, Hamilton, Ontario.
Export Citation:
APA/MLA Format     Download EndNote     Download BibTex
MeSH Terms
Adjuvants, Immunologic / pharmacology*
Antigens, CD3 / metabolism
Asthma / immunology*
CD4 Lymphocyte Count
CD8-Positive T-Lymphocytes / cytology
Cells, Cultured
Flow Cytometry
Hypersensitivity, Immediate / diagnosis,  immunology*
Interleukin-10 / biosynthesis*
Ionomycin / pharmacology
Leukocytes, Mononuclear / immunology
Lymphocyte Count
Methacholine Chloride / diagnostic use
T-Lymphocytes / immunology*,  metabolism*
Tetradecanoylphorbol Acetate / pharmacology
Reg. No./Substance:
0/Adjuvants, Immunologic; 0/Antigens, CD3; 130068-27-8/Interleukin-10; 16561-29-8/Tetradecanoylphorbol Acetate; 56092-81-0/Ionomycin; 62-51-1/Methacholine Chloride

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine

Previous Document:  Inhaled corticosteroids in COPD: determinants of use and trends in patient persistence with treatmen...
Next Document:  A population-based evaluation of a regional asthma education centre.