Document Detail

Different probe combinations for assessment of postzygotic chromosomal imbalances in human embryos.
MedLine Citation:
PMID:  12036085     Owner:  NLM     Status:  MEDLINE    
PURPOSE: We compared three different probe combinations for detection of postzygotic mosaic imbalances in human preimplantation embryos.
METHODS: Two hundred and two spare cleavage stage embryos were hybridized with fluorescently labelled DNA probe mixtures specific to chromosomes X, Y, 18 (N = 67), chromosomes 2, 7, 18 (N = 71), or chromosomes 13, 16, 18, 21, 22 (N = 64).
RESULTS: An overall higher incidence of abnormalities was detected using probe mixture for five (69%) or three (72%) autosomes compared to one autosome and chromosomes X and Y (54%). The rate of aneuploidy detected increased with the number of autosomes hybridized from 4% (X, Y, 18) to 11% (2, 7, 18) to 19% (13, 16, 18, 21, 22). Postzygotic mosaicism comprised the most frequent abnormality detected by all probe combinations, and the percentage detected by each was similar, 48% (X, Y, 18), 56% (2, 7,18), and 50% (13,16,18, 21, 22).
CONCLUSIONS: A probe combination of five autosomes, particularly those of clinical relevance, may be more beneficial for screening embryos from patients at risk of maternal-age-related aneuploidy. However, all three probe combinations are as efficient at identifying postzygotic mosaicism, and may be used for identifying embryos with less potential of developing to term.
Magdalena Bielanska; Seang Lin Tan; Asangla Ao
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Journal of assisted reproduction and genetics     Volume:  19     ISSN:  1058-0468     ISO Abbreviation:  J. Assist. Reprod. Genet.     Publication Date:  2002 Apr 
Date Detail:
Created Date:  2002-05-30     Completed Date:  2003-07-11     Revised Date:  2013-02-19    
Medline Journal Info:
Nlm Unique ID:  9206495     Medline TA:  J Assist Reprod Genet     Country:  United States    
Other Details:
Languages:  eng     Pagination:  177-82     Citation Subset:  IM    
Department of Obstetrics and Gynecology, Royal Victoria Hospital, McGill University, Montreal, Canada QC.
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MeSH Terms
Chromosome Aberrations*
Cleavage Stage, Ovum*
DNA Probes / diagnostic use*
Fertilization in Vitro
Genetic Testing
In Situ Hybridization, Fluorescence
Middle Aged
Mosaicism / genetics
Preimplantation Diagnosis*
Reg. No./Substance:
0/DNA Probes

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