| Different perforin expression in peripheral blood and prostate tissue in patients with benign prostatic hyperplasia and prostate cancer. | |
| | |
MedLine Citation:
|
PMID: 21535078 Owner: NLM Status: Publisher |
Abstract/OtherAbstract:
|
Perforin (P) is a prototypical cytotoxic molecule involved in cell-mediated immunity against various pathogens, alloantigens, and particularly different tumours. The purpose of this study was to determine P expression in different lymphocyte subpopulations isolated from peripheral blood and prostate tissue of patients with benign prostatic hyperplasia (BPH) and prostate cancer (PCa) and compare it with the P expression found in control group. Twenty subjects were recruited in each of the groups. Prostate mononuclear cells of the BPH and PCa tissues were isolated by enzymatic digestion and gradient density centrifugation, whereas peripheral blood mononuclear cells were isolated by gradient density centrifugation alone. Cells and tissue samples were labelled using monoclonal antibodies against P and different surface antigens (CD3, CD4, CD8, and CD56) and analysed by immunofluorescence and flow cytometry. Total P expression in peripheral blood lymphocytes did not differ significantly between BPH/PCa patients and control group, although the BPH and PCa tissue showed lower P expression level. A negative correlation between prostate-specific antigen (PSA) levels and the overall percentage of P(+) , CD3(+) CD56(-) P(+) , and CD3(-) CD56(+) P(+) cells in the prostate tissue was observed only in PCa patients. Our findings indicate that the low frequency of P(+) lymphocytes, including T, NKT, and NK cells, in the prostate tissue of patients with BPH and, particularly, PCa could be the consequence of local tissue microenvironment and one of the mechanisms involved in the pathogenesis of prostate hyperplasia following malignant alteration. |
| | |
Authors:
|
Vlatka Sotošek Tokmadžić; Maja Ilić Tomaš; Stanislav Sotošek; Gordana Laškarin; Marin Dominović; Vera Tulić; Gordana Ethorđević; Alan Sustić; Ines Mrakovčić-Šutić |
Related Documents
:
|
7283058 - Patient acceptance of mastectomy for cancer. 10077078 - Improving safety and aesthetic results in inverted t scar breast reduction. 15687668 - Endoscopic techniques in breast reconstruction. 17512298 - Immediate reconstruction using inframammary adipofascial flap of the anterior rectus sh... 21934688 - Effects of annual vs triennial mammography interval on breast cancer incidence and mort... 3132748 - Responsiveness of peripheral blood lymphocytes from cancer patients and healthy donors ... |
Publication Detail:
|
Type: JOURNAL ARTICLE Date: 2011-4-28 |
Journal Detail:
|
Title: Scandinavian journal of immunology Volume: - ISSN: 1365-3083 ISO Abbreviation: - Publication Date: 2011 Apr |
Date Detail:
|
Created Date: 2011-5-3 Completed Date: - Revised Date: - |
Medline Journal Info:
|
Nlm Unique ID: 0323767 Medline TA: Scand J Immunol Country: - |
Other Details:
|
Languages: ENG Pagination: - Citation Subset: - |
Copyright Information:
|
Copyright © 2011 Blackwell Publishing Ltd. |
Affiliation:
|
Department of Anaesthesiology, Reanimatology and Intensive Care, Medical Faculty, University of Rijeka, Rijeka, Croatia Department of Physiology and Immunology, Medical Faculty, University of Rijeka, Rijeka, Croatia Clinic of Urology, Clinical Hospital Centre Rijeka, Rijeka, Croatia Medical Faculty, University of Rijeka, Rijeka, Croatia Department of Pathology, Medical Faculty, University of Rijeka, Rijeka, Croatia. |
Export Citation:
|
APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
|
|
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
Previous Document: HLA class II alleles and chronic hepatitis C virus infection.
Next Document: WHAT FORMYL PEPTIDE RECEPTORS, IF ANY, ARE TRIGGERED BY COMPOUND 43 AND LIPOXIN A(4) ?